Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene
Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements that eliminated the repression of GFP gene expression induced by Alu and ORF2 and sequence chara...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Genética
2011-01-01
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Series: | Genetics and Molecular Biology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300005 |