Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene

Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements that eliminated the repression of GFP gene expression induced by Alu and ORF2 and sequence chara...

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Bibliographic Details
Main Authors: Honggang Wang, Wuzhuang Sun, Zhu Li, Xiufang Wang, Zhanjun Lv
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2011-01-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300005