Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors.
Neutrophils release fibrous traps of DNA, histones, and granule proteins known as neutrophil extracellular traps (NETs), which contribute to microbicidal killing and have been implicated in autoimmunity. The role of NET formation in the host response to nonbacterial pathogens is not well-understood....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4591979?pdf=render |
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| author | Daniel Sousa-Rocha Mariana Thomaz-Tobias Larissa Figueiredo Alves Diniz Priscila Silva Sampaio Souza Phileno Pinge-Filho Karina Alves Toledo |
| author_facet | Daniel Sousa-Rocha Mariana Thomaz-Tobias Larissa Figueiredo Alves Diniz Priscila Silva Sampaio Souza Phileno Pinge-Filho Karina Alves Toledo |
| author_sort | Daniel Sousa-Rocha |
| collection | DOAJ |
| description | Neutrophils release fibrous traps of DNA, histones, and granule proteins known as neutrophil extracellular traps (NETs), which contribute to microbicidal killing and have been implicated in autoimmunity. The role of NET formation in the host response to nonbacterial pathogens is not well-understood. In this study, we investigated the release of NETs by human neutrophils upon their interaction with Trypanosoma cruzi (Y strain) parasites. Our results showed that human neutrophils stimulated by T. cruzi generate NETs composed of DNA, histones, and elastase. The release occurred in a dose-, time-, and reactive oxygen species-dependent manner to decrease trypomastigote and increase amastigote numbers of the parasites without affecting their viability. NET release was decreased upon blocking with antibodies against Toll-like receptors 2 and 4. In addition, living parasites were not mandatory in the release of NETs induced by T. cruzi, as the same results were obtained when molecules from its soluble extract were tested. Our results increase the understanding of the stimulation of NETs by parasites, particularly T. cruzi. We suggest that contact of T. cruzi with NETs during Chagas's disease can limit infection by affecting the infectivity/pathogenicity of the parasite. |
| first_indexed | 2024-12-23T04:26:51Z |
| format | Article |
| id | doaj.art-babd942f7ecc4439b156de1a97b24c04 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T04:26:51Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-babd942f7ecc4439b156de1a97b24c042022-12-21T18:00:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e013956910.1371/journal.pone.0139569Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors.Daniel Sousa-RochaMariana Thomaz-TobiasLarissa Figueiredo Alves DinizPriscila Silva Sampaio SouzaPhileno Pinge-FilhoKarina Alves ToledoNeutrophils release fibrous traps of DNA, histones, and granule proteins known as neutrophil extracellular traps (NETs), which contribute to microbicidal killing and have been implicated in autoimmunity. The role of NET formation in the host response to nonbacterial pathogens is not well-understood. In this study, we investigated the release of NETs by human neutrophils upon their interaction with Trypanosoma cruzi (Y strain) parasites. Our results showed that human neutrophils stimulated by T. cruzi generate NETs composed of DNA, histones, and elastase. The release occurred in a dose-, time-, and reactive oxygen species-dependent manner to decrease trypomastigote and increase amastigote numbers of the parasites without affecting their viability. NET release was decreased upon blocking with antibodies against Toll-like receptors 2 and 4. In addition, living parasites were not mandatory in the release of NETs induced by T. cruzi, as the same results were obtained when molecules from its soluble extract were tested. Our results increase the understanding of the stimulation of NETs by parasites, particularly T. cruzi. We suggest that contact of T. cruzi with NETs during Chagas's disease can limit infection by affecting the infectivity/pathogenicity of the parasite.http://europepmc.org/articles/PMC4591979?pdf=render |
| spellingShingle | Daniel Sousa-Rocha Mariana Thomaz-Tobias Larissa Figueiredo Alves Diniz Priscila Silva Sampaio Souza Phileno Pinge-Filho Karina Alves Toledo Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors. PLoS ONE |
| title | Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors. |
| title_full | Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors. |
| title_fullStr | Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors. |
| title_full_unstemmed | Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors. |
| title_short | Trypanosoma cruzi and Its Soluble Antigens Induce NET Release by Stimulating Toll-Like Receptors. |
| title_sort | trypanosoma cruzi and its soluble antigens induce net release by stimulating toll like receptors |
| url | http://europepmc.org/articles/PMC4591979?pdf=render |
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