Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T Cells
Tissue-resident memory T (TRM) cells are immune sentinels that bear a key role in the local immune system and rapidly respond to infection. Our previous studies showed that mucosal immunization via intranasal pathways was more effective than intramuscular route. However, the mechanism of enhanced pr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/1403788 |
| _version_ | 1811190568030568448 |
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| author | Yangxue Ou Ying Wang Ting Yu Zhiyuan Cui Xin Chen Weijun Zhang Quanming Zou Jiang Gu Qianfei Zuo |
| author_facet | Yangxue Ou Ying Wang Ting Yu Zhiyuan Cui Xin Chen Weijun Zhang Quanming Zou Jiang Gu Qianfei Zuo |
| author_sort | Yangxue Ou |
| collection | DOAJ |
| description | Tissue-resident memory T (TRM) cells are immune sentinels that bear a key role in the local immune system and rapidly respond to infection. Our previous studies showed that mucosal immunization via intranasal pathways was more effective than intramuscular route. However, the mechanism of enhanced protective immunity remains unclear. Here, we formulated a Pseudomonas aeruginosa vaccine composed of type III secretion protein PcrV from P. aeruginosa and curdlan adjuvant and then administered by the intranasal route. Flow cytometry and immunofluorescence staining showed that the ratio of CD44+CD62L-CD69+CD4+ TRM cells induced by this vaccine was significantly increased, and IL-17A production was notably enhanced. Further analysis revealed that vaccinated mice can protect against the P. aeruginosa challenge even after administration with FTY720 treatment. What is more, our results showed that CD4+ TRM might be involved in the recruitment of neutrophils and provided partial protection against Pseudomonas aeruginosa. Taken together, these data demonstrated that CD4+ TRM cells were elicited in lung tissues after immunization with rePcrV and contributed to protective immunity. Furthermore, it provided novel strategies for the development of vaccines for P. aeruginosa and other respiratory-targeted vaccines. |
| first_indexed | 2024-04-11T14:53:08Z |
| format | Article |
| id | doaj.art-bae85e6df9f34c0ca8439a28a4d14dbd |
| institution | Directory Open Access Journal |
| issn | 2314-7156 |
| language | English |
| last_indexed | 2024-04-11T14:53:08Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj.art-bae85e6df9f34c0ca8439a28a4d14dbd2022-12-22T04:17:22ZengHindawi LimitedJournal of Immunology Research2314-71562022-01-01202210.1155/2022/1403788Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T CellsYangxue Ou0Ying Wang1Ting Yu2Zhiyuan Cui3Xin Chen4Weijun Zhang5Quanming Zou6Jiang Gu7Qianfei Zuo8Department of Microbiology and Biochemical Pharmacy953th HospitalDepartment of Microbiology and Biochemical PharmacyDepartment of Microbiology and Biochemical PharmacyDepartment of Microbiology and Biochemical PharmacyDepartment of Microbiology and Biochemical PharmacyDepartment of Microbiology and Biochemical PharmacyDepartment of Microbiology and Biochemical PharmacyDepartment of Microbiology and Biochemical PharmacyTissue-resident memory T (TRM) cells are immune sentinels that bear a key role in the local immune system and rapidly respond to infection. Our previous studies showed that mucosal immunization via intranasal pathways was more effective than intramuscular route. However, the mechanism of enhanced protective immunity remains unclear. Here, we formulated a Pseudomonas aeruginosa vaccine composed of type III secretion protein PcrV from P. aeruginosa and curdlan adjuvant and then administered by the intranasal route. Flow cytometry and immunofluorescence staining showed that the ratio of CD44+CD62L-CD69+CD4+ TRM cells induced by this vaccine was significantly increased, and IL-17A production was notably enhanced. Further analysis revealed that vaccinated mice can protect against the P. aeruginosa challenge even after administration with FTY720 treatment. What is more, our results showed that CD4+ TRM might be involved in the recruitment of neutrophils and provided partial protection against Pseudomonas aeruginosa. Taken together, these data demonstrated that CD4+ TRM cells were elicited in lung tissues after immunization with rePcrV and contributed to protective immunity. Furthermore, it provided novel strategies for the development of vaccines for P. aeruginosa and other respiratory-targeted vaccines.http://dx.doi.org/10.1155/2022/1403788 |
| spellingShingle | Yangxue Ou Ying Wang Ting Yu Zhiyuan Cui Xin Chen Weijun Zhang Quanming Zou Jiang Gu Qianfei Zuo Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T Cells Journal of Immunology Research |
| title | Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T Cells |
| title_full | Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T Cells |
| title_fullStr | Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T Cells |
| title_full_unstemmed | Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T Cells |
| title_short | Intranasal Vaccination with rePcrV Protects against Pseudomonas aeruginosa and Generates Lung Tissue-Resident Memory T Cells |
| title_sort | intranasal vaccination with repcrv protects against pseudomonas aeruginosa and generates lung tissue resident memory t cells |
| url | http://dx.doi.org/10.1155/2022/1403788 |
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