On Synthetic Instrument Response Functions of Time-Correlated Single-Photon Counting Based Fluorescence Lifetime Imaging Analysis

On Synthetic Instrument Response Functions of Time-Correlated Single-Photon Counting Based Fluorescence Lifetime Imaging Analysis

Time-correlated single-photon counting (TCSPC) has been the gold standard for fluorescence lifetime imaging (FLIM) techniques due to its high signal-to-noize ratio and high temporal resolution. The sensor system's temporal instrument response function (IRF) should be considered in the deconvolu...

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Bibliographic Details
Main Authors: Dong Xiao, Natakorn Sapermsap, Mohammed Safar, Margaret Rose Cunningham, Yu Chen, David Day‐Uei Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Physics
Subjects:
time-resolved imaging
photon counting
deconvolution
fluorescence microscopy
instrument response function
Online Access:https://www.frontiersin.org/articles/10.3389/fphy.2021.635645/full

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https://www.frontiersin.org/articles/10.3389/fphy.2021.635645/full

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