Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells
IntroductionProinflammatory cytokines are implicated in pancreatic ß cell failure in type 1 and type 2 diabetes and are known to stimulate alternative RNA splicing and the expression of nonsense-mediated RNA decay (NMD) components. Here, we investigate whether cytokines regulate NMD activity and ide...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2024.1359147/full |
| _version_ | 1797248704917274624 |
|---|---|
| author | Seyed M. Ghiasi Seyed M. Ghiasi Seyed M. Ghiasi Piero Marchetti Lorenzo Piemonti Jens H. Nielsen Bo T. Porse Bo T. Porse Bo T. Porse Thomas Mandrup-Poulsen Guy A. Rutter Guy A. Rutter Guy A. Rutter |
| author_facet | Seyed M. Ghiasi Seyed M. Ghiasi Seyed M. Ghiasi Piero Marchetti Lorenzo Piemonti Jens H. Nielsen Bo T. Porse Bo T. Porse Bo T. Porse Thomas Mandrup-Poulsen Guy A. Rutter Guy A. Rutter Guy A. Rutter |
| author_sort | Seyed M. Ghiasi |
| collection | DOAJ |
| description | IntroductionProinflammatory cytokines are implicated in pancreatic ß cell failure in type 1 and type 2 diabetes and are known to stimulate alternative RNA splicing and the expression of nonsense-mediated RNA decay (NMD) components. Here, we investigate whether cytokines regulate NMD activity and identify transcript isoforms targeted in ß cells.MethodsA luciferase-based NMD reporter transiently expressed in rat INS1(832/13), human-derived EndoC-ßH3, or dispersed human islet cells is used to examine the effect of proinflammatory cytokines (Cyt) on NMD activity. The gain- or loss-of-function of two key NMD components, UPF3B and UPF2, is used to reveal the effect of cytokines on cell viability and function. RNA-sequencing and siRNA-mediated silencing are deployed using standard techniques.ResultsCyt attenuate NMD activity in insulin-producing cell lines and primary human ß cells. These effects are found to involve ER stress and are associated with the downregulation of UPF3B. Increases or decreases in NMD activity achieved by UPF3B overexpression (OE) or UPF2 silencing raise or lower Cyt-induced cell death, respectively, in EndoC-ßH3 cells and are associated with decreased or increased insulin content, respectively. No effects of these manipulations are observed on glucose-stimulated insulin secretion. Transcriptomic analysis reveals that Cyt increases alternative splicing (AS)-induced exon skipping in the transcript isoforms, and this is potentiated by UPF2 silencing. Gene enrichment analysis identifies transcripts regulated by UPF2 silencing whose proteins are localized and/or functional in the extracellular matrix (ECM), including the serine protease inhibitor SERPINA1/α-1-antitrypsin, whose silencing sensitizes ß-cells to Cyt cytotoxicity. Cytokines suppress NMD activity via UPR signaling, potentially serving as a protective response against Cyt-induced NMD component expression.ConclusionOur findings highlight the central importance of RNA turnover in ß cell responses to inflammatory stress. |
| first_indexed | 2024-04-24T20:18:50Z |
| format | Article |
| id | doaj.art-bafcdcd0a3234dbca050690609495367 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-24T20:18:50Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-bafcdcd0a3234dbca0506906094953672024-03-22T10:56:20ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-03-011510.3389/fendo.2024.13591471359147Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cellsSeyed M. Ghiasi0Seyed M. Ghiasi1Seyed M. Ghiasi2Piero Marchetti3Lorenzo Piemonti4Jens H. Nielsen5Bo T. Porse6Bo T. Porse7Bo T. Porse8Thomas Mandrup-Poulsen9Guy A. Rutter10Guy A. Rutter11Guy A. Rutter12Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, United KingdomDepartment of Biomedical Sciences, University of Copenhagen, Copenhagen, DenmarkDevelopment and Aging Program, and Human Genetics Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United StatesDepartment of Clinical and Experimental Medicine, Islet Cell Laboratory, University of Pisa, Pisa, ItalyDiabetes Research Institute, Istituto Di Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale San Raffaele, Milano, ItalyDepartment of Biomedical Sciences, University of Copenhagen, Copenhagen, DenmarkBiotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen, DenmarkThe Finsen Laboratory, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen, DenmarkDanish Stem Cell Center (DanStem) Faculty of Health Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Biomedical Sciences, University of Copenhagen, Copenhagen, DenmarkSection of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, United KingdomCentre Hospitalier de l'Université de Montréal (CHUM) Research Centre (CRCHUM) and Faculty of Medicine, University of Montreal, Montreal, QC, Canada0Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, SingaporeIntroductionProinflammatory cytokines are implicated in pancreatic ß cell failure in type 1 and type 2 diabetes and are known to stimulate alternative RNA splicing and the expression of nonsense-mediated RNA decay (NMD) components. Here, we investigate whether cytokines regulate NMD activity and identify transcript isoforms targeted in ß cells.MethodsA luciferase-based NMD reporter transiently expressed in rat INS1(832/13), human-derived EndoC-ßH3, or dispersed human islet cells is used to examine the effect of proinflammatory cytokines (Cyt) on NMD activity. The gain- or loss-of-function of two key NMD components, UPF3B and UPF2, is used to reveal the effect of cytokines on cell viability and function. RNA-sequencing and siRNA-mediated silencing are deployed using standard techniques.ResultsCyt attenuate NMD activity in insulin-producing cell lines and primary human ß cells. These effects are found to involve ER stress and are associated with the downregulation of UPF3B. Increases or decreases in NMD activity achieved by UPF3B overexpression (OE) or UPF2 silencing raise or lower Cyt-induced cell death, respectively, in EndoC-ßH3 cells and are associated with decreased or increased insulin content, respectively. No effects of these manipulations are observed on glucose-stimulated insulin secretion. Transcriptomic analysis reveals that Cyt increases alternative splicing (AS)-induced exon skipping in the transcript isoforms, and this is potentiated by UPF2 silencing. Gene enrichment analysis identifies transcripts regulated by UPF2 silencing whose proteins are localized and/or functional in the extracellular matrix (ECM), including the serine protease inhibitor SERPINA1/α-1-antitrypsin, whose silencing sensitizes ß-cells to Cyt cytotoxicity. Cytokines suppress NMD activity via UPR signaling, potentially serving as a protective response against Cyt-induced NMD component expression.ConclusionOur findings highlight the central importance of RNA turnover in ß cell responses to inflammatory stress.https://www.frontiersin.org/articles/10.3389/fendo.2024.1359147/fullβ-cellsinsulin secretiontranscriptnonsense-mediated decayRNA decayRNA processing |
| spellingShingle | Seyed M. Ghiasi Seyed M. Ghiasi Seyed M. Ghiasi Piero Marchetti Lorenzo Piemonti Jens H. Nielsen Bo T. Porse Bo T. Porse Bo T. Porse Thomas Mandrup-Poulsen Guy A. Rutter Guy A. Rutter Guy A. Rutter Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells Frontiers in Endocrinology β-cells insulin secretion transcript nonsense-mediated decay RNA decay RNA processing |
| title | Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells |
| title_full | Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells |
| title_fullStr | Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells |
| title_full_unstemmed | Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells |
| title_short | Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells |
| title_sort | proinflammatory cytokines suppress nonsense mediated rna decay to impair regulated transcript isoform processing in pancreatic β cells |
| topic | β-cells insulin secretion transcript nonsense-mediated decay RNA decay RNA processing |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2024.1359147/full |
| work_keys_str_mv | AT seyedmghiasi proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT seyedmghiasi proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT seyedmghiasi proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT pieromarchetti proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT lorenzopiemonti proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT jenshnielsen proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT botporse proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT botporse proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT botporse proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT thomasmandruppoulsen proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT guyarutter proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT guyarutter proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells AT guyarutter proinflammatorycytokinessuppressnonsensemediatedrnadecaytoimpairregulatedtranscriptisoformprocessinginpancreaticbcells |