Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy
Abstract Dilated cardiomyopathy (DCM), as characterized by the left ventricular dilatation and contractile dysfunction, is one of the molecular mechanisms of which are largely unexplored. Here, we profiled the region‐resolved transcriptome and proteome of healthy and DCM human myocardial tissue and...
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Wiley
2023-02-01
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| Online Access: | https://doi.org/10.1002/VIW.20220040 |
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| author | Ling Lin Shanshan Liu Zhangwei Chen Yan Xia Juanjuan Xie Mingqiang Fu Danbo Lu Yuan Wu Huali Shen Pengyuan Yang Juying Qian |
| author_facet | Ling Lin Shanshan Liu Zhangwei Chen Yan Xia Juanjuan Xie Mingqiang Fu Danbo Lu Yuan Wu Huali Shen Pengyuan Yang Juying Qian |
| author_sort | Ling Lin |
| collection | DOAJ |
| description | Abstract Dilated cardiomyopathy (DCM), as characterized by the left ventricular dilatation and contractile dysfunction, is one of the molecular mechanisms of which are largely unexplored. Here, we profiled the region‐resolved transcriptome and proteome of healthy and DCM human myocardial tissue and obtained the deep‐coverage dataset consisting 7,605 proteins and 19,880 transcripts in four chambers of the human heart. On the basis of the core proteome and transcriptome characters of the healthy hearts, chamber‐specific proteome alterations were further revealed in end‐stage DCM, among which extracellular matrix (ECM), mitochondrial function, and muscle contraction were the most dysregulated biological processes. Protein–protein interaction network demonstrated divergent functional networks of DCM atrium and ventricle. Additionally, a 4‐biomarker panel (CTSB, vWF, C9, and MFGE8) was established with promising diagnostic potential for the DCM. Collectively, our data provide a global proteomic basis of the chamber‐specific cardiac tissue, and establish a protein catalog that holds promise for better definition and diagnosis of DCM. |
| first_indexed | 2024-04-10T08:55:58Z |
| format | Article |
| id | doaj.art-bb075c6763e24b08a91d180b65e9b6c3 |
| institution | Directory Open Access Journal |
| issn | 2688-3988 2688-268X |
| language | English |
| last_indexed | 2024-04-10T08:55:58Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
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| spelling | doaj.art-bb075c6763e24b08a91d180b65e9b6c32023-02-21T16:13:55ZengWileyView2688-39882688-268X2023-02-0141n/an/a10.1002/VIW.20220040Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathyLing Lin0Shanshan Liu1Zhangwei Chen2Yan Xia3Juanjuan Xie4Mingqiang Fu5Danbo Lu6Yuan Wu7Huali Shen8Pengyuan Yang9Juying Qian10Institutes of Biomedical Sciences of Shanghai Medical School & Minhang Hospital Fudan University Shanghai ChinaInstitutes of Biomedical Sciences of Shanghai Medical School & Minhang Hospital Fudan University Shanghai ChinaDepartment of Cardiology Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Fudan University Shanghai ChinaDepartment of Cardiology Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Fudan University Shanghai ChinaInstitutes of Biomedical Sciences of Shanghai Medical School & Minhang Hospital Fudan University Shanghai ChinaDepartment of Cardiology Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Fudan University Shanghai ChinaDepartment of Cardiology Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Fudan University Shanghai ChinaDepartment of Cardiology Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Fudan University Shanghai ChinaInstitutes of Biomedical Sciences of Shanghai Medical School & Minhang Hospital Fudan University Shanghai ChinaInstitutes of Biomedical Sciences of Shanghai Medical School & Minhang Hospital Fudan University Shanghai ChinaDepartment of Cardiology Shanghai Institute of Cardiovascular Diseases Zhongshan Hospital Fudan University Shanghai ChinaAbstract Dilated cardiomyopathy (DCM), as characterized by the left ventricular dilatation and contractile dysfunction, is one of the molecular mechanisms of which are largely unexplored. Here, we profiled the region‐resolved transcriptome and proteome of healthy and DCM human myocardial tissue and obtained the deep‐coverage dataset consisting 7,605 proteins and 19,880 transcripts in four chambers of the human heart. On the basis of the core proteome and transcriptome characters of the healthy hearts, chamber‐specific proteome alterations were further revealed in end‐stage DCM, among which extracellular matrix (ECM), mitochondrial function, and muscle contraction were the most dysregulated biological processes. Protein–protein interaction network demonstrated divergent functional networks of DCM atrium and ventricle. Additionally, a 4‐biomarker panel (CTSB, vWF, C9, and MFGE8) was established with promising diagnostic potential for the DCM. Collectively, our data provide a global proteomic basis of the chamber‐specific cardiac tissue, and establish a protein catalog that holds promise for better definition and diagnosis of DCM.https://doi.org/10.1002/VIW.20220040dilated cardiomyopathy, heart chambersproteome, serum biomarker panel, transcriptome |
| spellingShingle | Ling Lin Shanshan Liu Zhangwei Chen Yan Xia Juanjuan Xie Mingqiang Fu Danbo Lu Yuan Wu Huali Shen Pengyuan Yang Juying Qian Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy View dilated cardiomyopathy, heart chambers proteome, serum biomarker panel, transcriptome |
| title | Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy |
| title_full | Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy |
| title_fullStr | Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy |
| title_full_unstemmed | Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy |
| title_short | Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy |
| title_sort | anatomically resolved transcriptome and proteome landscapes reveal disease relevant molecular signatures and systematic changes in heart function of end stage dilated cardiomyopathy |
| topic | dilated cardiomyopathy, heart chambers proteome, serum biomarker panel, transcriptome |
| url | https://doi.org/10.1002/VIW.20220040 |
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