Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2

This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71∆CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were intranasally inoculated with 10<sup>5</sup...

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Main Authors: Joan Pujols, Elena Blázquez, Joaquim Segalés, Fernando Rodríguez, Chia-Yu Chang, Jordi Argilaguet, Laia Bosch-Camós, Rosa Rosell, Lola Pailler-García, Boris Gavrilov, Joy Campbell, Javier Polo
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Vaccines
Subjects:
Online Access:https://www.mdpi.com/2076-393X/11/4/825
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author Joan Pujols
Elena Blázquez
Joaquim Segalés
Fernando Rodríguez
Chia-Yu Chang
Jordi Argilaguet
Laia Bosch-Camós
Rosa Rosell
Lola Pailler-García
Boris Gavrilov
Joy Campbell
Javier Polo
author_facet Joan Pujols
Elena Blázquez
Joaquim Segalés
Fernando Rodríguez
Chia-Yu Chang
Jordi Argilaguet
Laia Bosch-Camós
Rosa Rosell
Lola Pailler-García
Boris Gavrilov
Joy Campbell
Javier Polo
author_sort Joan Pujols
collection DOAJ
description This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71∆CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were intranasally inoculated with 10<sup>5</sup> plaque-forming units (PFU) of live attenuated ASFV strain BA71∆CD2 and, three weeks later, left in direct contact with pigs infected with the pandemic Georgia 2007/01 ASFV strain. During the post-exposure (pe) period, 2/6 from the conventional diet group showed a transient peak rectal temperature >40.5 °C before day 20 pe, and some tissue samples collected at 20 d pe from 5/6 were PCR+ for ASFV, albeit showing Ct values much higher than Trojan pigs. Interestingly, the SDPP group did not show fever, neither PCR+ in blood nor rectal swab at any time pe, and none of the postmortem collected tissue samples were PCR+ for ASFV. Differential serum cytokine profiles among groups at vaccination, and a higher number of ASFV-specific IFNϒ-secreting T cells in pigs fed with SDPP soon after the Georgia 2007/01 encounter, confirmed the relevance of Th1-like responses in ASF protection. We believe that our result shows that nutritional interventions might contribute to improving future ASF vaccination strategies.
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spelling doaj.art-bb106bf662ab48198eb3f05ca49851782023-11-17T21:42:16ZengMDPI AGVaccines2076-393X2023-04-0111482510.3390/vaccines11040825Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2Joan Pujols0Elena Blázquez1Joaquim Segalés2Fernando Rodríguez3Chia-Yu Chang4Jordi Argilaguet5Laia Bosch-Camós6Rosa Rosell7Lola Pailler-García8Boris Gavrilov9Joy Campbell10Javier Polo11IRTA, Centre de Recerca en Sanitat Animal (CReSA), 08193 Barcelona, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA), 08193 Barcelona, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Barcelona, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA), 08193 Barcelona, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA), 08193 Barcelona, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA), 08193 Barcelona, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA), 08193 Barcelona, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Barcelona, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA), 08193 Barcelona, SpainBiologics Development, Huvepharma, 3A Nikolay Haytov Street, 1113 Sofia, BulgariaAPC, LLC, Ankeny, IA 50021, USAAPC Europe, S.L., 08403 Granollers, SpainThis study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71∆CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were intranasally inoculated with 10<sup>5</sup> plaque-forming units (PFU) of live attenuated ASFV strain BA71∆CD2 and, three weeks later, left in direct contact with pigs infected with the pandemic Georgia 2007/01 ASFV strain. During the post-exposure (pe) period, 2/6 from the conventional diet group showed a transient peak rectal temperature >40.5 °C before day 20 pe, and some tissue samples collected at 20 d pe from 5/6 were PCR+ for ASFV, albeit showing Ct values much higher than Trojan pigs. Interestingly, the SDPP group did not show fever, neither PCR+ in blood nor rectal swab at any time pe, and none of the postmortem collected tissue samples were PCR+ for ASFV. Differential serum cytokine profiles among groups at vaccination, and a higher number of ASFV-specific IFNϒ-secreting T cells in pigs fed with SDPP soon after the Georgia 2007/01 encounter, confirmed the relevance of Th1-like responses in ASF protection. We believe that our result shows that nutritional interventions might contribute to improving future ASF vaccination strategies.https://www.mdpi.com/2076-393X/11/4/825African swine feverASFVspray-dried porcine plasmavaccinechallengenutritional intervention
spellingShingle Joan Pujols
Elena Blázquez
Joaquim Segalés
Fernando Rodríguez
Chia-Yu Chang
Jordi Argilaguet
Laia Bosch-Camós
Rosa Rosell
Lola Pailler-García
Boris Gavrilov
Joy Campbell
Javier Polo
Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2
Vaccines
African swine fever
ASFV
spray-dried porcine plasma
vaccine
challenge
nutritional intervention
title Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2
title_full Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2
title_fullStr Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2
title_full_unstemmed Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2
title_short Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2
title_sort feeding spray dried porcine plasma to pigs improves the protection afforded by the african swine fever virus asfv ba71∆cd2 vaccine prototype against experimental challenge with the pandemic asfv study 2
topic African swine fever
ASFV
spray-dried porcine plasma
vaccine
challenge
nutritional intervention
url https://www.mdpi.com/2076-393X/11/4/825
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