Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats
Abstract Background Peel is one of the major by-products in fruit processing industry. Fruit products (non-edible parts) are also considered as waste products and often discarded in the environment. Fruit peels are now serving as one of the primary sources for isolation and extraction of secondary m...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-06-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s43088-020-00049-9 |
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| author | Sivasangari Ramya Vijayanand Narayanan Boomibalagan Ponnerulan Eswaran Saminathan Uthirapandi Veeranan |
| author_facet | Sivasangari Ramya Vijayanand Narayanan Boomibalagan Ponnerulan Eswaran Saminathan Uthirapandi Veeranan |
| author_sort | Sivasangari Ramya |
| collection | DOAJ |
| description | Abstract Background Peel is one of the major by-products in fruit processing industry. Fruit products (non-edible parts) are also considered as waste products and often discarded in the environment. Fruit peels are now serving as one of the primary sources for isolation and extraction of secondary metabolites in pharmaceutical industry. The present investigation was carried out to screen the phytochemical constituents and HPTLC analysis of peel extracts of Punica granatum and Citrus aurantifolia and their antidiabetic potential in alloxan-induced diabetic rats. Results Among the different solvent extracts, methanol solvent extract was found to possess more amounts of secondary metabolites. In addition, HPTLC analysis of the plant samples revealed the presence of 13 peaks in both the plants by using gallic acid as marker. Different biochemical parameters such as blood glucose, cholesterol, protein, urea, creatinine, and triglycerides level were subjected for estimation by collecting the blood samples from the treated diabetic rats after 21 days. A sharp decline in blood glucose, cholesterol, triglycerides, creatinine, and urea level was noticed when methanolic extracts of Punica granatum and Citrus aurantifolia were given to experimental animals when compared with negative control. However, protein and weight of the animal were found to be enhanced when treated with methanolic extracts of both the plants. Conclusion It can be concluded that fruit peels of both the plants exhibited antidiabetic potential on alloxan-induced diabetic rats which can be attributed to wide range of active pool of secondary metabolites. Further, screening and isolation of secondary metabolites along with their mode of action is required for effective use of plant-based drugs as antihyperglycemic agent. Graphical abstract |
| first_indexed | 2024-12-12T03:39:39Z |
| format | Article |
| id | doaj.art-bb1d0b13a3d04eb59bd7eeeda9561a16 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-12-12T03:39:39Z |
| publishDate | 2020-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-bb1d0b13a3d04eb59bd7eeeda9561a162022-12-22T00:39:43ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432020-06-019111110.1186/s43088-020-00049-9Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic ratsSivasangari Ramya0Vijayanand Narayanan1Boomibalagan Ponnerulan2Eswaran Saminathan3Uthirapandi Veeranan4Department of Biochemistry, Sri Sarada Niketen College for Women, Amaravathipudur, affiliated to Alagappa UniversityDepartment of Botany, Arumugam Pillai Seethai Ammal College, Thiruppathur, affiliated to Alagappa UniversityPG and Research Department of Botany, Raja Doraisingam Government Arts College, Sivagangai, affiliated to Alagappa UniversityPG and Research Department of Botany, Pasumpon Thiru Muthuramalingam Memorial College, Kamuthi, affiliated to Alagappa UniversityCentre for Research in Botany, Saraswathi Narayanan College Madurai, affiliated to Madurai Kamaraj UniversityAbstract Background Peel is one of the major by-products in fruit processing industry. Fruit products (non-edible parts) are also considered as waste products and often discarded in the environment. Fruit peels are now serving as one of the primary sources for isolation and extraction of secondary metabolites in pharmaceutical industry. The present investigation was carried out to screen the phytochemical constituents and HPTLC analysis of peel extracts of Punica granatum and Citrus aurantifolia and their antidiabetic potential in alloxan-induced diabetic rats. Results Among the different solvent extracts, methanol solvent extract was found to possess more amounts of secondary metabolites. In addition, HPTLC analysis of the plant samples revealed the presence of 13 peaks in both the plants by using gallic acid as marker. Different biochemical parameters such as blood glucose, cholesterol, protein, urea, creatinine, and triglycerides level were subjected for estimation by collecting the blood samples from the treated diabetic rats after 21 days. A sharp decline in blood glucose, cholesterol, triglycerides, creatinine, and urea level was noticed when methanolic extracts of Punica granatum and Citrus aurantifolia were given to experimental animals when compared with negative control. However, protein and weight of the animal were found to be enhanced when treated with methanolic extracts of both the plants. Conclusion It can be concluded that fruit peels of both the plants exhibited antidiabetic potential on alloxan-induced diabetic rats which can be attributed to wide range of active pool of secondary metabolites. Further, screening and isolation of secondary metabolites along with their mode of action is required for effective use of plant-based drugs as antihyperglycemic agent. Graphical abstracthttp://link.springer.com/article/10.1186/s43088-020-00049-9Fruit PeelPunica granatumCitrus aurantifoliaAntidiabetic activityGallic acidHPTLC analysis |
| spellingShingle | Sivasangari Ramya Vijayanand Narayanan Boomibalagan Ponnerulan Eswaran Saminathan Uthirapandi Veeranan Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats Beni-Suef University Journal of Basic and Applied Sciences Fruit Peel Punica granatum Citrus aurantifolia Antidiabetic activity Gallic acid HPTLC analysis |
| title | Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats |
| title_full | Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats |
| title_fullStr | Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats |
| title_full_unstemmed | Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats |
| title_short | Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats |
| title_sort | potential of peel extracts of punica granatum and citrus aurantifolia on alloxan induced diabetic rats |
| topic | Fruit Peel Punica granatum Citrus aurantifolia Antidiabetic activity Gallic acid HPTLC analysis |
| url | http://link.springer.com/article/10.1186/s43088-020-00049-9 |
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