Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves Applications
Hemocompatibility of cardiovascular implants represents a major clinical challenge and, to date, optimal antithrombotic properties are lacking. Next-generation tissue-engineered heart valves (TEHVs) made from human-cell-derived tissue-engineered extracellular matrices (hTEMs) demonstrated their rece...
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| Format: | Article |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.867877/full |
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| author | Sarah E. Motta Sarah E. Motta Polina Zaytseva Emanuela S. Fioretta Valentina Lintas Christian Breymann Simon P. Hoerstrup Simon P. Hoerstrup Maximilian Y. Emmert Maximilian Y. Emmert Maximilian Y. Emmert Maximilian Y. Emmert |
| author_facet | Sarah E. Motta Sarah E. Motta Polina Zaytseva Emanuela S. Fioretta Valentina Lintas Christian Breymann Simon P. Hoerstrup Simon P. Hoerstrup Maximilian Y. Emmert Maximilian Y. Emmert Maximilian Y. Emmert Maximilian Y. Emmert |
| author_sort | Sarah E. Motta |
| collection | DOAJ |
| description | Hemocompatibility of cardiovascular implants represents a major clinical challenge and, to date, optimal antithrombotic properties are lacking. Next-generation tissue-engineered heart valves (TEHVs) made from human-cell-derived tissue-engineered extracellular matrices (hTEMs) demonstrated their recellularization capacity in vivo and may represent promising candidates to avoid antithrombotic therapy. To further enhance their hemocompatibility, we tested hTEMs pre-endothelialization potential using human-blood-derived endothelial-colony-forming cells (ECFCs) and umbilical vein cells (control), cultured under static and dynamic orbital conditions, with either FBS or hPL. ECFCs performance was assessed via scratch assay, thereby recapitulating the surface damages occurring in transcatheter valves during crimping procedures. Our study demonstrated: feasibility to form a confluent and functional endothelium on hTEMs with expression of endothelium-specific markers; ECFCs migration and confluency restoration after crimping tests; hPL-induced formation of neo-microvessel-like structures; feasibility to pre-endothelialize hTEMs-based TEHVs and ECFCs retention on their surface after crimping. Our findings may stimulate new avenues towards next-generation pre-endothelialized implants with enhanced hemocompatibility, being beneficial for selected high-risk patients. |
| first_indexed | 2024-04-12T22:45:06Z |
| format | Article |
| id | doaj.art-bb371a46890042029971b368d4c98ce5 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T22:45:06Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-bb371a46890042029971b368d4c98ce52022-12-22T03:13:35ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.867877867877Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves ApplicationsSarah E. Motta0Sarah E. Motta1Polina Zaytseva2Emanuela S. Fioretta3Valentina Lintas4Christian Breymann5Simon P. Hoerstrup6Simon P. Hoerstrup7Maximilian Y. Emmert8Maximilian Y. Emmert9Maximilian Y. Emmert10Maximilian Y. Emmert11Institute for Regenerative Medicine (IREM), University of Zurich, Zurich, SwitzerlandWyss Translational Center Zurich, University and ETH Zurich, Zurich, SwitzerlandInstitute for Regenerative Medicine (IREM), University of Zurich, Zurich, SwitzerlandInstitute for Regenerative Medicine (IREM), University of Zurich, Zurich, SwitzerlandInstitute for Regenerative Medicine (IREM), University of Zurich, Zurich, SwitzerlandDepartment of Obstetrics and Gynaecology, University Hospital Zurich, Obstetric Research, Feto- Maternal Haematology Research Group, Zurich, SwitzerlandInstitute for Regenerative Medicine (IREM), University of Zurich, Zurich, SwitzerlandWyss Translational Center Zurich, University and ETH Zurich, Zurich, SwitzerlandInstitute for Regenerative Medicine (IREM), University of Zurich, Zurich, SwitzerlandWyss Translational Center Zurich, University and ETH Zurich, Zurich, SwitzerlandDepartment of Cardiovascular Surgery, Charité Universitätsmedizin Berlin, Berlin, GermanyDepartment of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, GermanyHemocompatibility of cardiovascular implants represents a major clinical challenge and, to date, optimal antithrombotic properties are lacking. Next-generation tissue-engineered heart valves (TEHVs) made from human-cell-derived tissue-engineered extracellular matrices (hTEMs) demonstrated their recellularization capacity in vivo and may represent promising candidates to avoid antithrombotic therapy. To further enhance their hemocompatibility, we tested hTEMs pre-endothelialization potential using human-blood-derived endothelial-colony-forming cells (ECFCs) and umbilical vein cells (control), cultured under static and dynamic orbital conditions, with either FBS or hPL. ECFCs performance was assessed via scratch assay, thereby recapitulating the surface damages occurring in transcatheter valves during crimping procedures. Our study demonstrated: feasibility to form a confluent and functional endothelium on hTEMs with expression of endothelium-specific markers; ECFCs migration and confluency restoration after crimping tests; hPL-induced formation of neo-microvessel-like structures; feasibility to pre-endothelialize hTEMs-based TEHVs and ECFCs retention on their surface after crimping. Our findings may stimulate new avenues towards next-generation pre-endothelialized implants with enhanced hemocompatibility, being beneficial for selected high-risk patients.https://www.frontiersin.org/articles/10.3389/fbioe.2022.867877/fullhuman cell-derived tissue-engineered matricesendothelial colony forming cellanti-coagulationhemocomaptibilityscratch assayHPL |
| spellingShingle | Sarah E. Motta Sarah E. Motta Polina Zaytseva Emanuela S. Fioretta Valentina Lintas Christian Breymann Simon P. Hoerstrup Simon P. Hoerstrup Maximilian Y. Emmert Maximilian Y. Emmert Maximilian Y. Emmert Maximilian Y. Emmert Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves Applications Frontiers in Bioengineering and Biotechnology human cell-derived tissue-engineered matrices endothelial colony forming cell anti-coagulation hemocomaptibility scratch assay HPL |
| title | Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves Applications |
| title_full | Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves Applications |
| title_fullStr | Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves Applications |
| title_full_unstemmed | Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves Applications |
| title_short | Endothelial Progenitor Cell-Based in vitro Pre-Endothelialization of Human Cell-Derived Biomimetic Regenerative Matrices for Next-Generation Transcatheter Heart Valves Applications |
| title_sort | endothelial progenitor cell based in vitro pre endothelialization of human cell derived biomimetic regenerative matrices for next generation transcatheter heart valves applications |
| topic | human cell-derived tissue-engineered matrices endothelial colony forming cell anti-coagulation hemocomaptibility scratch assay HPL |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.867877/full |
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