An Image Analysis Pipeline for Quantifying the Features of Fluorescently-Labeled Biomolecular Condensates in Cells

An Image Analysis Pipeline for Quantifying the Features of Fluorescently-Labeled Biomolecular Condensates in Cells

Biomolecular condensates are cellular organelles formed through liquid-liquid phase separation (LLPS) that play critical roles in cellular functions including signaling, transcription, translation, and stress response. Importantly, condensate misregulation is associated with human diseases, includin...

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Bibliographic Details
Main Authors: David W. Baggett, Anna Medyukhina, Swarnendu Tripathi, Hazheen K. Shirnekhi, Huiyun Wu, Stanley B. Pounds, Khaled Khairy, Richard Kriwacki
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Bioinformatics
Subjects:
fluorescence microscopy
biomolecular condensate
liquid-liquid phase separation
image analysis
puncta features
open-source software
Online Access:https://www.frontiersin.org/articles/10.3389/fbinf.2022.897238/full

Internet

https://www.frontiersin.org/articles/10.3389/fbinf.2022.897238/full

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