Label-free measurement of the yeast short chain TAG lipase activity by ESI-MS after one-step esterification

Label-free measurement of the yeast short chain TAG lipase activity by ESI-MS after one-step esterification

Triacylglycerol (TAG) lipases hydrolyze ester bonds in TAG and release diacylglycerol (DAG), monoacylglycerol (MAG), and FA. We present a one-step chemical derivatization method for label-free quantification of a mixture of TAG, DAG, and MAG following lipase assay by ESI-MS. Because the ionization e...

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Bibliographic Details
Main Authors: Hye Jin Ham, Jongcheol Seo, Hye-Joo Yoon, Seung Koo Shin
Format: Article
Language:English
Published: Elsevier 2017-03-01
Series:Journal of Lipid Research
Subjects:
chemical derivatization
triacylglycerol
lipolysis
specific activity
electrospray ionization-mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520304569

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http://www.sciencedirect.com/science/article/pii/S0022227520304569

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