Free DNA and Metagenomics Analyses: Evaluation of Free DNA Inactivation Protocols for Shotgun Metagenomics Analysis of Human Biological Matrices

Free DNA and Metagenomics Analyses: Evaluation of Free DNA Inactivation Protocols for Shotgun Metagenomics Analysis of Human Biological Matrices

Culture-independent approaches now represent the gold standard for the investigation of both environmental and host-associated complex microbial communities. Nevertheless, despite the great advantages offered by these novel methodologies based on the use of next-generation DNA sequencing approaches,...

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Bibliographic Details
Main Authors: Leonardo Mancabelli, Christian Milani, Rosaria Anzalone, Giulia Alessandri, Gabriele Andrea Lugli, Chiara Tarracchini, Federico Fontana, Francesca Turroni, Marco Ventura
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-10-01
Series:Frontiers in Microbiology
Subjects:
propidium monoazide
free DNA
shotgun metagenomics
human microbiome
real-time PCR
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.749373/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.749373/full

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