The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats
Objective: To investigate the effects of quercetin (Q) and rutin on 5-fluorouracil (5-FU)-induced hepatotoxicity. Methods: The control group was corn oil. The 5-FU group rats were corn oil and injected intraperitoneal 5-FU 50 mg/kg. Groups rutin 50 + 5-FU and rutin 100 + 5-FU were respectively 50 mg...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-07-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169117305762 |
| _version_ | 1818949655976738816 |
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| author | Volkan Gelen Emin Şengül Semin Gedikli Gözde Atila Hamit Uslu Mustafa Makav |
| author_facet | Volkan Gelen Emin Şengül Semin Gedikli Gözde Atila Hamit Uslu Mustafa Makav |
| author_sort | Volkan Gelen |
| collection | DOAJ |
| description | Objective: To investigate the effects of quercetin (Q) and rutin on 5-fluorouracil (5-FU)-induced hepatotoxicity.
Methods: The control group was corn oil. The 5-FU group rats were corn oil and injected intraperitoneal 5-FU 50 mg/kg. Groups rutin 50 + 5-FU and rutin 100 + 5-FU were respectively 50 mg/kg and 100 mg/kg rutin. These groups were given 5-FU (50 mg/kg) in the 18th day. The group rutin 100 was rutin (100 mg/kg i.g.). Groups Q50 + 5-FU and Q100 + 5-FU were respectively 50 mg/kg and 100 mg/kg quercetin. These groups were given 5-FU (50 mg/kg) in the 18th day of quercetin application. The group Q100 was quercetin (100 mg/kg i.g.). In the end of experimental applications, blood was collected from anesthetized rats.
Results: The MDA level was significantly higher in the 5-FU group compared with control group, and determined to be decreased in other groups. GPx and GSH levels were significantly decreased in the 5-FU group compared to the control, rutin 100 + 5-FU and Q100 + 5-FU groups. AST, ALT, LDH and ALP levels in the serum were significantly increased in the 5-FU group compared with the other groups. The results from this analysis show that while the caspase-3 level increases in the 5-FU group, it decreases in the Q50 + 5-FU, Q100 + 5-FU, rutin 50 + 5-FU and rutin 100 + 5-FU groups. Bcl-2 level decreased in the 5-FU group compared to the control group, but increased in the rutin 100 + 5-FU, Q50 + 5-FU and Q100 + 5-FU groups.
Conclusions: In this study it was determined that the rutin and Q have protective effects on 5-FU-induced hepatotoxicity. |
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| id | doaj.art-bbc4cf0912e24165b4faefbb6a0754fa |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-20T09:06:09Z |
| publishDate | 2017-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-bbc4cf0912e24165b4faefbb6a0754fa2022-12-21T19:45:43ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-07-017764765310.1016/j.apjtb.2017.06.013The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in ratsVolkan Gelen0Emin Şengül1Semin Gedikli2Gözde Atila3Hamit Uslu4Mustafa Makav5Department of Physiology, Veterinary Faculty, Kafkas University, Kars, TurkeyDepartment of Physiology, Veterinary Faculty, Atatürk University, Erzurum, TurkeyDepartment of Histology-Embryology, Veterinary Faculty, Atatürk University, Erzurum, TurkeyDepartment of Physiology, Veterinary Faculty, Kafkas University, Kars, TurkeyDepartment of Health Care Services, Atatürk Health Vocational School, Kafkas University, Kars, TurkeyDepartment of Physiology, Veterinary Faculty, Kafkas University, Kars, TurkeyObjective: To investigate the effects of quercetin (Q) and rutin on 5-fluorouracil (5-FU)-induced hepatotoxicity. Methods: The control group was corn oil. The 5-FU group rats were corn oil and injected intraperitoneal 5-FU 50 mg/kg. Groups rutin 50 + 5-FU and rutin 100 + 5-FU were respectively 50 mg/kg and 100 mg/kg rutin. These groups were given 5-FU (50 mg/kg) in the 18th day. The group rutin 100 was rutin (100 mg/kg i.g.). Groups Q50 + 5-FU and Q100 + 5-FU were respectively 50 mg/kg and 100 mg/kg quercetin. These groups were given 5-FU (50 mg/kg) in the 18th day of quercetin application. The group Q100 was quercetin (100 mg/kg i.g.). In the end of experimental applications, blood was collected from anesthetized rats. Results: The MDA level was significantly higher in the 5-FU group compared with control group, and determined to be decreased in other groups. GPx and GSH levels were significantly decreased in the 5-FU group compared to the control, rutin 100 + 5-FU and Q100 + 5-FU groups. AST, ALT, LDH and ALP levels in the serum were significantly increased in the 5-FU group compared with the other groups. The results from this analysis show that while the caspase-3 level increases in the 5-FU group, it decreases in the Q50 + 5-FU, Q100 + 5-FU, rutin 50 + 5-FU and rutin 100 + 5-FU groups. Bcl-2 level decreased in the 5-FU group compared to the control group, but increased in the rutin 100 + 5-FU, Q50 + 5-FU and Q100 + 5-FU groups. Conclusions: In this study it was determined that the rutin and Q have protective effects on 5-FU-induced hepatotoxicity.http://www.sciencedirect.com/science/article/pii/S2221169117305762Rat5-FluorouracilQuercetinRutinHepatotoxicity |
| spellingShingle | Volkan Gelen Emin Şengül Semin Gedikli Gözde Atila Hamit Uslu Mustafa Makav The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats Asian Pacific Journal of Tropical Biomedicine Rat 5-Fluorouracil Quercetin Rutin Hepatotoxicity |
| title | The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats |
| title_full | The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats |
| title_fullStr | The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats |
| title_full_unstemmed | The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats |
| title_short | The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats |
| title_sort | protective effect of rutin and quercetin on 5 fu induced hepatotoxicity in rats |
| topic | Rat 5-Fluorouracil Quercetin Rutin Hepatotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2221169117305762 |
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