An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages
Histoplasma capsulatum is responsible for a human systemic mycosis that primarily affects lung tissue. Macrophages are the major effector cells in humans that respond to the fungus, and the development of respiratory disease depends on the ability of Histoplasma yeast cells to survive and replicate...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01526/full |
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| author | Nayla De Souza Pitangui Janaina De Cássia Orlandi Sardi Aline Raquel eVoltan Claudia Tavares edos Santos Julhiany De Fátima Da Silva Rosângela Aparecida Moraes da Silva Felipe Oliveira Souza Christiane Pienna Soares Gabriela eRodríguez-Arellanes Maria Lúcia Taylor Maria José Soares Mendes-Giannini Ana Marisa eFusco-Almeida |
| author_facet | Nayla De Souza Pitangui Janaina De Cássia Orlandi Sardi Aline Raquel eVoltan Claudia Tavares edos Santos Julhiany De Fátima Da Silva Rosângela Aparecida Moraes da Silva Felipe Oliveira Souza Christiane Pienna Soares Gabriela eRodríguez-Arellanes Maria Lúcia Taylor Maria José Soares Mendes-Giannini Ana Marisa eFusco-Almeida |
| author_sort | Nayla De Souza Pitangui |
| collection | DOAJ |
| description | Histoplasma capsulatum is responsible for a human systemic mycosis that primarily affects lung tissue. Macrophages are the major effector cells in humans that respond to the fungus, and the development of respiratory disease depends on the ability of Histoplasma yeast cells to survive and replicate within alveolar macrophages. Therefore, the interaction between macrophages and H. capsulatum is a decisive step in the yeast dissemination into host tissues. Although the role played by components of cell-mediated immunity in the host's defense system and the mechanisms used by the pathogen to evade the host immune response are well understood, knowledge regarding the effects induced by H. capsulatum in host cells at the nuclear level is limited. According to the present findings, H. capsulatum yeast cells display a unique architectural arrangement during the intracellular infection of cultured murine alveolar macrophages, characterized as a formation of aggregates that seem to surround the host cell nucleus, resembling a crown. This extranuclear organization of yeast-aggregates generates damage on the nucleus of the host cell, producing DNA fragmentation and inducing apoptosis, even though the yeast cells are not located inside the nucleus and do not trigger changes in nuclear proteins. The current study highlights a singular intracellular arrangement of H. capsulatum yeast near to the nucleus of infected murine alveolar macrophages that may contribute to the yeast’s persistence under intracellular conditions, since this fungal pathogen may display different strategies to prevent elimination by the host's phagocytic mechanisms. |
| first_indexed | 2024-12-21T22:44:25Z |
| format | Article |
| id | doaj.art-bbd8b3641b2b40ef8fd15682eb0dac1f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T22:44:25Z |
| publishDate | 2016-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bbd8b3641b2b40ef8fd15682eb0dac1f2022-12-21T18:47:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-01-01610.3389/fmicb.2015.01526167413An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophagesNayla De Souza Pitangui0Janaina De Cássia Orlandi Sardi1Aline Raquel eVoltan2Claudia Tavares edos Santos3Julhiany De Fátima Da Silva4Rosângela Aparecida Moraes da Silva5Felipe Oliveira Souza6Christiane Pienna Soares7Gabriela eRodríguez-Arellanes8Maria Lúcia Taylor9Maria José Soares Mendes-Giannini10Ana Marisa eFusco-Almeida11UNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNAM - Universidad Nacional Autónoma de México, Departamento de Microbiologia y Parasitologia, Facultad de MedicinaUNAM - Universidad Nacional Autónoma de México, Departamento de Microbiologia y Parasitologia, Facultad de MedicinaUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasUNESP - Univ Estadual Paulista, Departamento de Análises Clínicas, Faculdade de Ciências FarmacêuticasHistoplasma capsulatum is responsible for a human systemic mycosis that primarily affects lung tissue. Macrophages are the major effector cells in humans that respond to the fungus, and the development of respiratory disease depends on the ability of Histoplasma yeast cells to survive and replicate within alveolar macrophages. Therefore, the interaction between macrophages and H. capsulatum is a decisive step in the yeast dissemination into host tissues. Although the role played by components of cell-mediated immunity in the host's defense system and the mechanisms used by the pathogen to evade the host immune response are well understood, knowledge regarding the effects induced by H. capsulatum in host cells at the nuclear level is limited. According to the present findings, H. capsulatum yeast cells display a unique architectural arrangement during the intracellular infection of cultured murine alveolar macrophages, characterized as a formation of aggregates that seem to surround the host cell nucleus, resembling a crown. This extranuclear organization of yeast-aggregates generates damage on the nucleus of the host cell, producing DNA fragmentation and inducing apoptosis, even though the yeast cells are not located inside the nucleus and do not trigger changes in nuclear proteins. The current study highlights a singular intracellular arrangement of H. capsulatum yeast near to the nucleus of infected murine alveolar macrophages that may contribute to the yeast’s persistence under intracellular conditions, since this fungal pathogen may display different strategies to prevent elimination by the host's phagocytic mechanisms.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01526/fullHost-Pathogen InteractionsHistoplasma capsulatumnucleusalveolar macrophagesintracellular arrangement |
| spellingShingle | Nayla De Souza Pitangui Janaina De Cássia Orlandi Sardi Aline Raquel eVoltan Claudia Tavares edos Santos Julhiany De Fátima Da Silva Rosângela Aparecida Moraes da Silva Felipe Oliveira Souza Christiane Pienna Soares Gabriela eRodríguez-Arellanes Maria Lúcia Taylor Maria José Soares Mendes-Giannini Ana Marisa eFusco-Almeida An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages Frontiers in Microbiology Host-Pathogen Interactions Histoplasma capsulatum nucleus alveolar macrophages intracellular arrangement |
| title | An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
| title_full | An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
| title_fullStr | An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
| title_full_unstemmed | An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
| title_short | An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
| title_sort | intracellular arrangement of histoplasma capsulatum yeast aggregates generates nuclear damage to the cultured murine alveolar macrophages |
| topic | Host-Pathogen Interactions Histoplasma capsulatum nucleus alveolar macrophages intracellular arrangement |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01526/full |
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