Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue

Loop-mediated isothermal amplification (LAMP), a novel gene amplification method, enables the synthesis of larger amounts of both DNA and a visible byproduct—namely, magnesium pyrophosphate—without thermal cycling. A positive reaction is indicated by the turbidity of the reaction solution or the col...

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Bibliographic Details
Main Authors: Motoki Goto, Eiichi Honda, Atsuo Ogura, Akio Nomoto, Ken-Ichi Hanaki
Format: Article
Language:English
Published: Taylor & Francis Group 2009-03-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000113072