Biophysical analysis of Gaussia luciferase bioluminescence mechanisms using a non-oxidizable coelenterazine

Biophysical analysis of Gaussia luciferase bioluminescence mechanisms using a non-oxidizable coelenterazine

Gaussia luciferase (GLuc 18.2kDa; 168 residues) is a marine copepod luciferase that emits a bright blue light when oxidizing coelenterazine (CTZ). It is a helical protein where two homologous sequential repeats form two anti-parallel bundles, each made of four helices. We previously identified a hyd...

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Bibliographic Details
Main Authors: Kyoko Takatsu, Naohiro Kobayashi, Nan Wu, Yves L. Janin, Toshio Yamazaki, Yutaka Kuroda
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:BBA Advances
Subjects:
SEP-tag, hydrophobic cavity
Intrinsically disordered region (IDR)
NMR
Coelenterazine
Azacoelenterazine
Chemical shifts
Online Access:http://www.sciencedirect.com/science/article/pii/S266716032200028X

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http://www.sciencedirect.com/science/article/pii/S266716032200028X

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