Effective RNA Knockdown Using CRISPR-Cas13a and Molecular Targeting of the <i>EML4-ALK</i> Transcript in H3122 Lung Cancer Cells

Effective RNA Knockdown Using CRISPR-Cas13a and Molecular Targeting of the <i>EML4-ALK</i> Transcript in H3122 Lung Cancer Cells

RNAi technology has significant potential as a future therapeutic and could theoretically be used to knock down disease-specific RNAs. However, due to frequent off-target effects, low efficiency, and limited accessibility of nuclear transcripts, the clinical application of the technology remains cha...

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Bibliographic Details
Main Authors: Saifullah, Matomo Sakari, Takeshi Suzuki, Seiji Yano, Toshifumi Tsukahara
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:International Journal of Molecular Sciences
Subjects:
RNA stability
RNA knockdown
CRISPR-Cas13a
firefly luciferase
EML4-ALK
cell viability
Online Access:https://www.mdpi.com/1422-0067/21/23/8904

Internet

https://www.mdpi.com/1422-0067/21/23/8904

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