Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple Sclerosis
Multiple sclerosis (MS) is an immune-driven demyelinating disease of the central nervous system. Immune cell features are particularly promising as predictive biomarkers due to their central role in the pathogenesis but also as drug targets, even if nowadays, they have no impact in clinical practice...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.653577/full |
| _version_ | 1819138268652896256 |
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| author | Laura Couloume Juliette Ferrant Simon Le Gallou Simon Le Gallou Marion Mandon Marion Mandon Rachel Jean Rachel Jean Nadège Bescher Nadège Bescher Helene Zephir Gilles Edan Eric Thouvenot Eric Thouvenot Aurelie Ruet Aurelie Ruet Aurelie Ruet Marc Debouverie Marc Debouverie Karin Tarte Karin Tarte Patricia Amé Patricia Amé Mikael Roussel Mikael Roussel Laure Michel Laure Michel Laure Michel |
| author_facet | Laura Couloume Juliette Ferrant Simon Le Gallou Simon Le Gallou Marion Mandon Marion Mandon Rachel Jean Rachel Jean Nadège Bescher Nadège Bescher Helene Zephir Gilles Edan Eric Thouvenot Eric Thouvenot Aurelie Ruet Aurelie Ruet Aurelie Ruet Marc Debouverie Marc Debouverie Karin Tarte Karin Tarte Patricia Amé Patricia Amé Mikael Roussel Mikael Roussel Laure Michel Laure Michel Laure Michel |
| author_sort | Laura Couloume |
| collection | DOAJ |
| description | Multiple sclerosis (MS) is an immune-driven demyelinating disease of the central nervous system. Immune cell features are particularly promising as predictive biomarkers due to their central role in the pathogenesis but also as drug targets, even if nowadays, they have no impact in clinical practice. Recently, high-resolution approaches, such as mass cytometry (CyTOF), helped to better understand the diversity and functions of the immune system. In this study, we performed an exploratory analysis of blood immune response profiles in healthy controls and MS patients sampled at their first neurological relapse, using two large CyTOF panels including 62 markers exploring myeloid and lymphoid cells. An increased abundance of both a T-bet-expressing B cell subset and a CD206+ classical monocyte subset was detected in the blood of early MS patients. Moreover, T-bet-expressing B cells tended to be enriched in aggressive MS patients. This study provides new insights into understanding the pathophysiology of MS and the identification of immunological biomarkers. Further studies will be required to validate these results and to determine the exact role of the identified clusters in neuroinflammation. |
| first_indexed | 2024-12-22T11:04:04Z |
| format | Article |
| id | doaj.art-bc2d8e837ec74af998627a4cd8b329ab |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T11:04:04Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-bc2d8e837ec74af998627a4cd8b329ab2022-12-21T18:28:23ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-05-011210.3389/fimmu.2021.653577653577Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple SclerosisLaura Couloume0Juliette Ferrant1Simon Le Gallou2Simon Le Gallou3Marion Mandon4Marion Mandon5Rachel Jean6Rachel Jean7Nadège Bescher8Nadège Bescher9Helene Zephir10Gilles Edan11Eric Thouvenot12Eric Thouvenot13Aurelie Ruet14Aurelie Ruet15Aurelie Ruet16Marc Debouverie17Marc Debouverie18Karin Tarte19Karin Tarte20Patricia Amé21Patricia Amé22Mikael Roussel23Mikael Roussel24Laure Michel25Laure Michel26Laure Michel27INSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceCHU Lille, CRCSEP Lille, Univ Lille, Lille, FranceNeurology Department, Rennes Clinical Investigation Centre, Rennes University Hospital-Rennes University-INSERM, Rennes, FranceDepartment of Neurology, Nimes University Hospital, Nimes, FranceInstitut de Génomique Fonctionnelle, UMR5203, Inserm 1191, Université de Montpellier, Montpellier, FranceUniversité de Bordeaux, Bordeaux, FranceNeurocentre Magendie, INSERM U1215, Bordeaux, FranceCHU de Bordeaux, Department of Neurology, Bordeaux, France0Nancy University Hospital, Department of Neurology, Nancy, France1Université de Lorraine, APEMAC, Nancy, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceINSERM, Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-CHU Rennes, 2 rue Henri Le Guilloux, Rennes, FranceNeurology Department, Rennes Clinical Investigation Centre, Rennes University Hospital-Rennes University-INSERM, Rennes, FranceMultiple sclerosis (MS) is an immune-driven demyelinating disease of the central nervous system. Immune cell features are particularly promising as predictive biomarkers due to their central role in the pathogenesis but also as drug targets, even if nowadays, they have no impact in clinical practice. Recently, high-resolution approaches, such as mass cytometry (CyTOF), helped to better understand the diversity and functions of the immune system. In this study, we performed an exploratory analysis of blood immune response profiles in healthy controls and MS patients sampled at their first neurological relapse, using two large CyTOF panels including 62 markers exploring myeloid and lymphoid cells. An increased abundance of both a T-bet-expressing B cell subset and a CD206+ classical monocyte subset was detected in the blood of early MS patients. Moreover, T-bet-expressing B cells tended to be enriched in aggressive MS patients. This study provides new insights into understanding the pathophysiology of MS and the identification of immunological biomarkers. Further studies will be required to validate these results and to determine the exact role of the identified clusters in neuroinflammation.https://www.frontiersin.org/articles/10.3389/fimmu.2021.653577/fullbiomarkermultiple sclerosismass cytometryimmunologyB cellsmonocytes |
| spellingShingle | Laura Couloume Juliette Ferrant Simon Le Gallou Simon Le Gallou Marion Mandon Marion Mandon Rachel Jean Rachel Jean Nadège Bescher Nadège Bescher Helene Zephir Gilles Edan Eric Thouvenot Eric Thouvenot Aurelie Ruet Aurelie Ruet Aurelie Ruet Marc Debouverie Marc Debouverie Karin Tarte Karin Tarte Patricia Amé Patricia Amé Mikael Roussel Mikael Roussel Laure Michel Laure Michel Laure Michel Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple Sclerosis Frontiers in Immunology biomarker multiple sclerosis mass cytometry immunology B cells monocytes |
| title | Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple Sclerosis |
| title_full | Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple Sclerosis |
| title_fullStr | Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple Sclerosis |
| title_full_unstemmed | Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple Sclerosis |
| title_short | Mass Cytometry Identifies Expansion of T-bet+ B Cells and CD206+ Monocytes in Early Multiple Sclerosis |
| title_sort | mass cytometry identifies expansion of t bet b cells and cd206 monocytes in early multiple sclerosis |
| topic | biomarker multiple sclerosis mass cytometry immunology B cells monocytes |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.653577/full |
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