Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memory
Abstract Aim Active changes in neuronal DNA methylation and demethylation appear to act as controllers of synaptic scaling and glutamate receptor trafficking in learning and memory formation. DNA methyltransferases (DNMTs), including proteins encoded by Dnmt1, Dnmt3a and Dnmt3b, are dominant enzymes...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13041-020-00574-9 |
| _version_ | 1819050040709087232 |
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| author | Qingnuan Kong Ming Yu Meng Zhang Chuang Wei Huating Gu Shaoyang Yu Wei Sun Nan Li Yu Zhou |
| author_facet | Qingnuan Kong Ming Yu Meng Zhang Chuang Wei Huating Gu Shaoyang Yu Wei Sun Nan Li Yu Zhou |
| author_sort | Qingnuan Kong |
| collection | DOAJ |
| description | Abstract Aim Active changes in neuronal DNA methylation and demethylation appear to act as controllers of synaptic scaling and glutamate receptor trafficking in learning and memory formation. DNA methyltransferases (DNMTs), including proteins encoded by Dnmt1, Dnmt3a and Dnmt3b, are dominant enzymes carrying out DNA methylation. Our previous study demonstrated the important roles that DNMT1 and DNMT3a play in synaptic function and memory. In this study, we aim to explore the role of DNMT3b and its-mediated DNA methylation in memory processes. Methods Dnmt3b was knocked down specifically in dorsal CA1 neurons of adult mice hippocampus by AAV-syn-Cre-GFP virus injection. Behavioral tests were used to evaluate memory performance. Gene expression microarray analysis followed by quantitative RT-PCR were performed to find differential expression genes. Results Dnmt3b flox/flox mice receiving Cre-virus infection showed impaired novel object-place recognition (NPR) and normal novel object recognition (NOR), in comparison to mice receiving control GFP-virus infection. Microarray analysis revealed differential expression of K+ channel subunits in the hippocampus of Dnmt3b flox/flox mice receiving Cre-virus injection. Increased Kcne2 expression was confirmed by following qRT-PCR analysis. We also found that NPR training and testing induced up-regulation of hippocampal Dnmt1 and Dnmt3a mRNA expression in control mice, but not in Cre-virus injected mice. Our findings thus demonstrate that conditional Dnmt3b deletion in a sub-region of the hippocampus impairs a specific form of recognition memory that is hippocampus-dependent. |
| first_indexed | 2024-12-21T11:41:44Z |
| format | Article |
| id | doaj.art-bc3cea64c4384d0fb077863881944c4a |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-21T11:41:44Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-bc3cea64c4384d0fb077863881944c4a2022-12-21T19:05:17ZengBMCMolecular Brain1756-66062020-03-011311410.1186/s13041-020-00574-9Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memoryQingnuan Kong0Ming Yu1Meng Zhang2Chuang Wei3Huating Gu4Shaoyang Yu5Wei Sun6Nan Li7Yu Zhou8Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Qingdao UniversityAbstract Aim Active changes in neuronal DNA methylation and demethylation appear to act as controllers of synaptic scaling and glutamate receptor trafficking in learning and memory formation. DNA methyltransferases (DNMTs), including proteins encoded by Dnmt1, Dnmt3a and Dnmt3b, are dominant enzymes carrying out DNA methylation. Our previous study demonstrated the important roles that DNMT1 and DNMT3a play in synaptic function and memory. In this study, we aim to explore the role of DNMT3b and its-mediated DNA methylation in memory processes. Methods Dnmt3b was knocked down specifically in dorsal CA1 neurons of adult mice hippocampus by AAV-syn-Cre-GFP virus injection. Behavioral tests were used to evaluate memory performance. Gene expression microarray analysis followed by quantitative RT-PCR were performed to find differential expression genes. Results Dnmt3b flox/flox mice receiving Cre-virus infection showed impaired novel object-place recognition (NPR) and normal novel object recognition (NOR), in comparison to mice receiving control GFP-virus infection. Microarray analysis revealed differential expression of K+ channel subunits in the hippocampus of Dnmt3b flox/flox mice receiving Cre-virus injection. Increased Kcne2 expression was confirmed by following qRT-PCR analysis. We also found that NPR training and testing induced up-regulation of hippocampal Dnmt1 and Dnmt3a mRNA expression in control mice, but not in Cre-virus injected mice. Our findings thus demonstrate that conditional Dnmt3b deletion in a sub-region of the hippocampus impairs a specific form of recognition memory that is hippocampus-dependent.http://link.springer.com/article/10.1186/s13041-020-00574-9Dnmt3bMemoryHippocampusObject-place recognition |
| spellingShingle | Qingnuan Kong Ming Yu Meng Zhang Chuang Wei Huating Gu Shaoyang Yu Wei Sun Nan Li Yu Zhou Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memory Molecular Brain Dnmt3b Memory Hippocampus Object-place recognition |
| title | Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memory |
| title_full | Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memory |
| title_fullStr | Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memory |
| title_full_unstemmed | Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memory |
| title_short | Conditional Dnmt3b deletion in hippocampal dCA1 impairs recognition memory |
| title_sort | conditional dnmt3b deletion in hippocampal dca1 impairs recognition memory |
| topic | Dnmt3b Memory Hippocampus Object-place recognition |
| url | http://link.springer.com/article/10.1186/s13041-020-00574-9 |
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