Carrier molecules and extraction of circulating tumor DNA for next generation sequencing in colorectal cancer
The aims of the study were: i) to compare circulating tumor DNA (ctDNA) yields obtained by different manual extraction procedures, ii) to evaluate the addition of various carrier molecules into the plasma to improve ctDNA extraction recovery, and iii) to use next generation sequencing (NGS) technolo...
Main Authors: | Martin Beránek, Igor Sirák, Milan Vošmik, Jiří Petera, Monika Drastíková, Vladimír Palička |
---|---|
Format: | Article |
Language: | English |
Published: |
Karolinum Press
2016-08-01
|
Series: | Acta Medica |
Subjects: | |
Online Access: | http://actamedica.lfhk.cuni.cz/59/2/0054/ |
Similar Items
-
Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma
by: Martin Beránek, et al.
Published: (2011-01-01) -
Polyadenylated Sequencing Primers Enable Complete Readability of PCR Amplicons Analyzed by Dideoxynucleotide Sequencing
by: Martin Beránek, et al.
Published: (2012-01-01) -
Advances in CO2 circulation hydrogen carriers and catalytic processes
by: Seo Ono, et al.
Published: (2024-02-01) -
Postoperative Circulating Tumor DNA Can Predict High Risk Patients with Colorectal Cancer Based on Next-Generation Sequencing
by: Chul Seung Lee, et al.
Published: (2021-08-01) -
Comparison of variant allele frequency and number of mutant molecules as units of measurement for circulating tumor DNA
by: Manouk K. Bos, et al.
Published: (2021-01-01)