Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Summary: Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene’s effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 tech...

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Bibliografiske detaljer
Main Authors: Anne Huber, Christine Dijkstra, Matthias Ernst, Moritz F. Eissmann
Format: Article
Sprog:English
Udgivet: Elsevier 2023-03-01
Serier:STAR Protocols
Fag:
Cancer
CRISPR
Organoids
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166723000345

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723000345

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