Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Summary: Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene’s effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 tech...

Mô tả đầy đủ

Chi tiết về thư mục
Những tác giả chính:	Anne Huber, Christine Dijkstra, Matthias Ernst, Moritz F. Eissmann
Định dạng:	Bài viết
Ngôn ngữ:	English
Được phát hành:	Elsevier 2023-03-01
Loạt:	STAR Protocols
Những chủ đề:	Cancer CRISPR Organoids
Truy cập trực tuyến:	http://www.sciencedirect.com/science/article/pii/S2666166723000345

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