Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9

Summary: Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene’s effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 tech...

Disgrifiad llawn

Manylion Llyfryddiaeth
Prif Awduron: Anne Huber, Christine Dijkstra, Matthias Ernst, Moritz F. Eissmann
Fformat: Erthygl
Iaith:English
Cyhoeddwyd: Elsevier 2023-03-01
Cyfres:STAR Protocols
Pynciau:
Cancer
CRISPR
Organoids
Mynediad Ar-lein:http://www.sciencedirect.com/science/article/pii/S2666166723000345

Rhyngrwyd

http://www.sciencedirect.com/science/article/pii/S2666166723000345

Eitemau Tebyg