Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9
Summary: Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene’s effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 tech...
| Autori principali: | , , , |
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| Natura: | Articolo |
| Lingua: | English |
| Pubblicazione: |
Elsevier
2023-03-01
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| Serie: | STAR Protocols |
| Soggetti: | |
| Accesso online: | http://www.sciencedirect.com/science/article/pii/S2666166723000345 |