Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünal
Format: Artikel
Sprache:English
Veröffentlicht: Elsevier 2022-03-01
Schriftenreihe:STAR Protocols
Schlagworte:
Bioinformatics
Sequence analysis
Genetics
Genomics
Sequencing
Model Organisms
Online Zugang:http://www.sciencedirect.com/science/article/pii/S2666166722000259

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http://www.sciencedirect.com/science/article/pii/S2666166722000259

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