Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...

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Detalhes bibliográficos
Main Authors: Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünal
Formato: Artigo
Idioma:English
Publicado em: Elsevier 2022-03-01
Colecção:STAR Protocols
Assuntos:
Bioinformatics
Sequence analysis
Genetics
Genomics
Sequencing
Model Organisms
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166722000259

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722000259

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