Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...

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Detaylı Bibliyografya
Asıl Yazarlar: Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünal
Materyal Türü: Makale
Dil:English
Baskı/Yayın Bilgisi: Elsevier 2022-03-01
Seri Bilgileri:STAR Protocols
Konular:
Bioinformatics
Sequence analysis
Genetics
Genomics
Sequencing
Model Organisms
Online Erişim:http://www.sciencedirect.com/science/article/pii/S2666166722000259

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722000259

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