The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species
The trypsin-like peptidase activity assay kit measures the trypsin-like protease produced by three red-complex species, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i>, and <i>Treponema denticola</i>, causing periodontitis, and detects the presence of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/12/9/2172 |
| _version_ | 1827661872473571328 |
|---|---|
| author | Michihiko Usui Masanori Iwasaki Wataru Ariyoshi Kaoru Kobayashi Shingo Kasai Rieko Yamanaka Keisuke Nakashima Tatsuji Nishihara |
| author_facet | Michihiko Usui Masanori Iwasaki Wataru Ariyoshi Kaoru Kobayashi Shingo Kasai Rieko Yamanaka Keisuke Nakashima Tatsuji Nishihara |
| author_sort | Michihiko Usui |
| collection | DOAJ |
| description | The trypsin-like peptidase activity assay kit measures the trypsin-like protease produced by three red-complex species, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i>, and <i>Treponema denticola</i>, causing periodontitis, and detects the presence of these bacteria in samples. The purpose of this study was to investigate the relationship between the detection of TLPs by a novel TLP-AA, ADCHECK and the detection of red-complex pathogens by real-time PCR using tongue swabs from patients with periodontitis. The detection limit of trypsin-like protease activity by ADCHECK was validated using the culture supernatants of two different <i>Porphyromonas gingivalis</i> bacterial strains. Real-time PCR was performed to determine the number of red-complex species in the tongue coatings of patients with periodontal disease. Trypsin-like protease activity in tongue-swab samples was scored using ADCHECK. ADCHECK successfully detected trypsin-like protease activity in 10<sup>3</sup><i> Porphyromonas gingivalis</i> bacterial strains. The specificity, positive predictive value, negative predictive value, and accuracy of ADCHECK for the presence of red-complex pathogens determined by real-time PCR were 90%, 97%, 98%, and 92%, respectively. ADCHECK is an effective tool for the detection of red-complex pathogens. |
| first_indexed | 2024-03-10T00:17:15Z |
| format | Article |
| id | doaj.art-bcb9bc9568ca4023991bcbe076d3e8a8 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T00:17:15Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-bcb9bc9568ca4023991bcbe076d3e8a82023-11-23T15:49:54ZengMDPI AGDiagnostics2075-44182022-09-01129217210.3390/diagnostics12092172The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex SpeciesMichihiko Usui0Masanori Iwasaki1Wataru Ariyoshi2Kaoru Kobayashi3Shingo Kasai4Rieko Yamanaka5Keisuke Nakashima6Tatsuji Nishihara7Division of Periodontology, Department of Oral Function, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, JapanTokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-Ku, Tokyo 173-0015, JapanDivision of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu 803-8580, JapanDivision of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Kitakyushu 803-8580, JapanDivision of Periodontology, Department of Oral Function, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, JapanDivision of Periodontology, Department of Oral Function, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, JapanDivision of Periodontology, Department of Oral Function, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, JapanDivision of Periodontal Medicine, Kyushu Dental University, Kitakyushu 803-8580, JapanThe trypsin-like peptidase activity assay kit measures the trypsin-like protease produced by three red-complex species, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i>, and <i>Treponema denticola</i>, causing periodontitis, and detects the presence of these bacteria in samples. The purpose of this study was to investigate the relationship between the detection of TLPs by a novel TLP-AA, ADCHECK and the detection of red-complex pathogens by real-time PCR using tongue swabs from patients with periodontitis. The detection limit of trypsin-like protease activity by ADCHECK was validated using the culture supernatants of two different <i>Porphyromonas gingivalis</i> bacterial strains. Real-time PCR was performed to determine the number of red-complex species in the tongue coatings of patients with periodontal disease. Trypsin-like protease activity in tongue-swab samples was scored using ADCHECK. ADCHECK successfully detected trypsin-like protease activity in 10<sup>3</sup><i> Porphyromonas gingivalis</i> bacterial strains. The specificity, positive predictive value, negative predictive value, and accuracy of ADCHECK for the presence of red-complex pathogens determined by real-time PCR were 90%, 97%, 98%, and 92%, respectively. ADCHECK is an effective tool for the detection of red-complex pathogens.https://www.mdpi.com/2075-4418/12/9/2172trypsin-like peptidaseperiodontitisred complex |
| spellingShingle | Michihiko Usui Masanori Iwasaki Wataru Ariyoshi Kaoru Kobayashi Shingo Kasai Rieko Yamanaka Keisuke Nakashima Tatsuji Nishihara The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species Diagnostics trypsin-like peptidase periodontitis red complex |
| title | The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species |
| title_full | The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species |
| title_fullStr | The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species |
| title_full_unstemmed | The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species |
| title_short | The Ability of a Novel Trypsin-like Peptidase Activity Assay Kit to Detect Red-Complex Species |
| title_sort | ability of a novel trypsin like peptidase activity assay kit to detect red complex species |
| topic | trypsin-like peptidase periodontitis red complex |
| url | https://www.mdpi.com/2075-4418/12/9/2172 |
| work_keys_str_mv | AT michihikousui theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT masanoriiwasaki theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT wataruariyoshi theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT kaorukobayashi theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT shingokasai theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT riekoyamanaka theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT keisukenakashima theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT tatsujinishihara theabilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT michihikousui abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT masanoriiwasaki abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT wataruariyoshi abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT kaorukobayashi abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT shingokasai abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT riekoyamanaka abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT keisukenakashima abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies AT tatsujinishihara abilityofanoveltrypsinlikepeptidaseactivityassaykittodetectredcomplexspecies |