Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection

Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection

Molecular diagnostic testing for viral pathogens is crucial in aquaculture. The efficient and convenient preparation of pathogenic microbial nucleic acids is the basis of molecular diagnosis. Here, we developed a simplified deoxyribonucleic acid (DNA) extraction method from aquatic animal DNA viruse...

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Bibliographic Details
Main Authors: Xi Hu, Nan Jiang, Yiqun Li, Yong Zhou, Yuding Fan, Mingyang Xue, Lingbing Zeng, Wenzhi Liu, Yan Meng
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Animals
Subjects:
Chelex 100 resin
deoxyribonucleic acid preparation
aquatic animal virus
detection
Online Access:https://www.mdpi.com/2076-2615/12/15/1999

Internet

https://www.mdpi.com/2076-2615/12/15/1999

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