MicroScale Thermophoresis (MST) for studying actin polymerization kinetics

Here, we present a MicroScale Thermophoresis (MST)–based assay for in vitro assessment of actin polymerization. By monitoring the thermophoretic behavior of ATTO488-labeled actin in a temperature gradient over time, we could follow polymerization in real time and resolve its three characteristic pha...

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Bibliographic Details
Main Authors: Andrea Topf, Peter Franz, Georgios Tsiavaliaris
Format: Article
Language:English
Published: Taylor & Francis Group 2017-10-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000114599