MAPK Phosphorylation Assay with Leaf Disks of Arabidopsis

Activation of mitogen activated protein kinases (MAPKs) is involved in many abiotic and biotic stress responses including plant defense. MAPK acitvation is based on the dual phosphorylation of threonine (T) and tyrosin (Y) residues (T-x-Y motif) in the activation loop of the MAPK protein. By determi...

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Bibliographic Details
Main Authors: Pascale Flury, Dominik Klauser, Thomas Boller, Sebastian Bartels
Format: Article
Language:English
Published: Bio-protocol LLC 2013-10-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=929&type=0