Protocol for quantifying SA-β-gal activity as a measure of senescence in islets of a mouse model of type 1 diabetes

Protocol for quantifying SA-β-gal activity as a measure of senescence in islets of a mouse model of type 1 diabetes

Summary: A subpopulation of pancreatic beta cells becomes senescent during type 1 diabetes (T1D) progression, and removal of these populations protects against T1D in mice. Here, we present a protocol to measure senescence in murine pancreatic islet cells through analysis of senescence-associated β-...

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Bibliographic Details
Main Authors: Hugo Lee, Peter J. Thompson, Feyza Engin
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Metabolism
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000881

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http://www.sciencedirect.com/science/article/pii/S2666166724000881

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