Extended two-photon microscopy in live samples with Bessel beams: steadier focus, faster volume scans, and simpler stereoscopic imaging

Extended two-photon microscopy in live samples with Bessel beams: steadier focus, faster volume scans, and simpler stereoscopic imaging

Two-photon microscopy has revolutionized functional cellular imaging in tissue, but although the highly confined depth of field of standard set-ups yields great optical sectioning, it also limits imaging speed and ease of use. For this reason, we recently presented a simple and retrofittable modific...

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Bibliographic Details
Main Authors: Gabrielle eThériault, Martin eCottet, Annie eCastonguay, Nathalie eMcCarthy, Yves eDe Koninck
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-05-01
Series:Frontiers in Cellular Neuroscience
Subjects:
Cellular Imaging
3D imaging
temporal resolution
depth of field
nonlinear microscopy
axicon
Online Access:http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00139/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00139/full

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