Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysis

Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysis

Summary: Here, we present a protocol for exploring the effects of PPP1R15A inhibitor, Sephin1, on antitumor immunity of B16F1 subcutaneous tumor in mice. We describe steps for constructing single-cell transcriptome and TCR libraries, sequencing, and using sequencing data for the integration of expre...

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Bibliographic Details
Main Authors: Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu, Ling-Hua Meng, Jingyu Lang, Gang Jin, Yichuan Xiao, Landian Hu, Xiangyin Kong
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Bioinformatics
Cancer
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672300583X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672300583X

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