High-capacity adenovector delivery of forced CRISPR-Cas9 heterodimers fosters precise chromosomal deletions in human cells

High-capacity adenovector delivery of forced CRISPR-Cas9 heterodimers fosters precise chromosomal deletions in human cells

Genome editing based on dual CRISPR-Cas9 complexes (multiplexes) permits removing specific genomic sequences in living cells leveraging research on functional genomics and genetic therapies. Delivering the required large and multicomponent reagents in a synchronous and stoichiometric manner remains,...

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Bibliographic Details
Main Authors: Francesca Tasca, Marcella Brescia, Jin Liu, Josephine M. Janssen, Kamel Mamchaoui, Manuel A.F.V. Gonçalves
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA Editing
multiplexing gene editing
gene delivery
fusion Cas9 proteins
high-capacity adenoviral vectors
on-target specificity
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253123000458

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http://www.sciencedirect.com/science/article/pii/S2162253123000458

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