A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum

A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum

The lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed...

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Bibliographic Details
Main Authors: Baojie eJiang, Ruiqin eZhang, Dan eFeng, Fangzhong eWang, Kuimei eLiu, Yi eJiang, Kangle eNiu, Quanquan eYuan, Mingyu eWang, Hailong eWang, Youming eZhang, Xu eFang
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-04-01
Series:Frontiers in Microbiology
Subjects:
Doxycycline
Cre-loxP system
Penicillium oxalicum
Tet-On system
Selective markers
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00485/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00485/full

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