A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum
The lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00485/full |
| _version_ | 1818367765384265728 |
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| author | Baojie eJiang Ruiqin eZhang Dan eFeng Fangzhong eWang Kuimei eLiu Yi eJiang Kangle eNiu Quanquan eYuan Mingyu eWang Hailong eWang Hailong eWang Youming eZhang Youming eZhang Xu eFang |
| author_facet | Baojie eJiang Ruiqin eZhang Dan eFeng Fangzhong eWang Kuimei eLiu Yi eJiang Kangle eNiu Quanquan eYuan Mingyu eWang Hailong eWang Hailong eWang Youming eZhang Youming eZhang Xu eFang |
| author_sort | Baojie eJiang |
| collection | DOAJ |
| description | The lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed a genetic manipulation system taking advantage of two established genetic systems: the Cre-loxP system and Tet-on system in Penicillium oxalicum JUA10-1. This system is simple and efficient. The expression of Cre recombinase was activated by doxycycline since it was controlled by Tet-on system. Using this system, two genes, ligD and bglI, were sequentially disrupted by loxP flanked ptrA. The successful application of this procedure will provide a useful tool for genetic engineering in filamentous fungi. This system will also play an important role in improving the productivity of interesting products and minimizing by-product when fermented by filamentous fungi. |
| first_indexed | 2024-12-13T22:57:15Z |
| format | Article |
| id | doaj.art-bd4f8618c0ee4bd799c3b900acd3fd24 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T22:57:15Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bd4f8618c0ee4bd799c3b900acd3fd242022-12-21T23:28:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-04-01710.3389/fmicb.2016.00485183596A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicumBaojie eJiang0Ruiqin eZhang1Dan eFeng2Fangzhong eWang3Kuimei eLiu4Yi eJiang5Kangle eNiu6Quanquan eYuan7Mingyu eWang8Hailong eWang9Hailong eWang10Youming eZhang11Youming eZhang12Xu eFang13Shandong UniversityShandong UniversityShandong UniversityShandong UniversityShandong UniversityShandong UniversityShandong UniversityShandong UniversityShandong UniversityShandong UniversityShandong University-Helmholtz Institute of BiotechnologyShandong UniversityShandong University-Helmholtz Institute of BiotechnologyShandong UniversityThe lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed a genetic manipulation system taking advantage of two established genetic systems: the Cre-loxP system and Tet-on system in Penicillium oxalicum JUA10-1. This system is simple and efficient. The expression of Cre recombinase was activated by doxycycline since it was controlled by Tet-on system. Using this system, two genes, ligD and bglI, were sequentially disrupted by loxP flanked ptrA. The successful application of this procedure will provide a useful tool for genetic engineering in filamentous fungi. This system will also play an important role in improving the productivity of interesting products and minimizing by-product when fermented by filamentous fungi.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00485/fullDoxycyclineCre-loxP systemPenicillium oxalicumTet-On systemSelective markers |
| spellingShingle | Baojie eJiang Ruiqin eZhang Dan eFeng Fangzhong eWang Kuimei eLiu Yi eJiang Kangle eNiu Quanquan eYuan Mingyu eWang Hailong eWang Hailong eWang Youming eZhang Youming eZhang Xu eFang A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum Frontiers in Microbiology Doxycycline Cre-loxP system Penicillium oxalicum Tet-On system Selective markers |
| title | A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum |
| title_full | A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum |
| title_fullStr | A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum |
| title_full_unstemmed | A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum |
| title_short | A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum |
| title_sort | tet on and cre loxp based genetic engineering system for convenient recycling of selection markers in penicillium oxalicum |
| topic | Doxycycline Cre-loxP system Penicillium oxalicum Tet-On system Selective markers |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00485/full |
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