Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system

Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system

Abstract Background The hemolysin (Hly) secretion system of E. coli allows the one-step translocation of hemolysin A (HlyA) from the bacterial cytoplasm to the extracellular medium, without a periplasmic intermediate. In this work, we investigate whether the Hly secretion system of E. coli is compet...

Full description

Bibliographic Details
Main Authors: David Ruano-Gallego, Sofía Fraile, Carlos Gutierrez, Luis Ángel Fernández
Format: Article
Language:English
Published: BMC 2019-03-01
Series:Microbial Cell Factories
Subjects:
E. coli/hemolysin
Nanobodies
Protein secretion
Single-domain antibodies
Online Access:http://link.springer.com/article/10.1186/s12934-019-1094-0

Internet

http://link.springer.com/article/10.1186/s12934-019-1094-0

Similar Items