Phosphosite Scanning reveals a complex phosphorylation code underlying CDK-dependent activation of Hcm1

Many kinases phosphorylate proteins on multiple sites, however in most cases it is not known which sites are functionally important. Here, the authors describe a high-throughput approach to quantitatively evaluate the contribution of each phosphosite within a multisite phosphorylated domain.

Bibliographic Details
Main Authors: Michelle M. Conti, Rui Li, Michelle A. Narváez Ramos, Lihua Julie Zhu, Thomas G. Fazzio, Jennifer A. Benanti
Format: Article
Language:English
Published: Nature Portfolio 2023-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-36035-9