Two dimensional blue native/SDS-PAGE to identify mitochondrial complex I subunits modified by 4-hydroxynonenal (HNE)

Two dimensional blue native/SDS-PAGE to identify mitochondrial complex I subunits modified by 4-hydroxynonenal (HNE)

The lipid peroxidation product 4-hydroxynonenal (HNE) can form protein-linked HNE adducts, thereby impacting protein structure and function. Mitochondrial complex I (NADH-ubiquinone oxidoreductase), containing at least 45 subunits in mammalian cells, sits in a lipid-rich environment and is thus very...

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Bibliographic Details
Main Authors: Jinzi eWu, Xiaoting eLuo, Liang-Jun eYan
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-03-01
Series:Frontiers in Physiology
Subjects:
Mitochondria
Reactive Oxygen Species
diabetes
Streptozotocin
4-hydroxynonenal
Blue native/SDS-PAGE
Online Access:http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00098/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00098/full

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