Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction
Background/Purpose: Cancer stem cells (CSCs) are deemed as the driving force of tumorigenesis in oral squamous cell carcinomas (OSCCs). In this study, we investigated the chemotherapeutic effect of sulforaphane, a dietary component from broccoli sprouts, on targeting OSCC-CSCs. Methods: The effect o...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664616000280 |
| _version_ | 1818844490465542144 |
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| author | Chia-Ming Liu Chih-Yu Peng Yi-Wen Liao Ming-Yi Lu Meng-Lun Tsai Jung-Chun Yeh Chuan-Hang Yu Cheng-Chia Yu |
| author_facet | Chia-Ming Liu Chih-Yu Peng Yi-Wen Liao Ming-Yi Lu Meng-Lun Tsai Jung-Chun Yeh Chuan-Hang Yu Cheng-Chia Yu |
| author_sort | Chia-Ming Liu |
| collection | DOAJ |
| description | Background/Purpose: Cancer stem cells (CSCs) are deemed as the driving force of tumorigenesis in oral squamous cell carcinomas (OSCCs). In this study, we investigated the chemotherapeutic effect of sulforaphane, a dietary component from broccoli sprouts, on targeting OSCC-CSCs.
Methods: The effect of sulforaphane on normal oral epithelial cells (SG) and sphere-forming OSCC-CSCs isolated from SAS and GNM cells was examined. ALDH1 activity and CD44 positivity of OSCC-CSCs with sulforaphane treatment was assessed by flow cytometry analysis. In vitro and in vivo tumorigenicity assays of OSCC-CSCs with sulforaphane treatment were presented.
Results: We observed that the sulforaphane dose-dependently eliminated the proliferation rate of OSCC-CSCs, whereas the inhibition on SG cells proliferation was limited. Cancer stemness properties including self-renewal, CD44 positivity, and ALDH1 activity were also decreased in OSCC-CSCs with different doses of sulforaphane treatment. Moreover, sulforaphane treatment of OSCC-CSCs decreased the migration, invasion, clonogenicity, and in vivo tumorigenicity of xenograghts. Sulforaphane treatment resulted in a dose-dependent increase in the levels of tumor suppressive miR200c.
Conclusion: These lines of evidence suggest that sulforaphane can suppress the cancer stemness and tumor-initiating properties in OSCC-CSCs both in vitro and in vivo. |
| first_indexed | 2024-12-19T05:14:36Z |
| format | Article |
| id | doaj.art-bd5c779079ad4895ba9ea0ee08a8f580 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-19T05:14:36Z |
| publishDate | 2017-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-bd5c779079ad4895ba9ea0ee08a8f5802022-12-21T20:34:43ZengElsevierJournal of the Formosan Medical Association0929-66462017-01-011161414810.1016/j.jfma.2016.01.004Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c inductionChia-Ming Liu0Chih-Yu Peng1Yi-Wen Liao2Ming-Yi Lu3Meng-Lun Tsai4Jung-Chun Yeh5Chuan-Hang Yu6Cheng-Chia Yu7School of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, TaiwanBackground/Purpose: Cancer stem cells (CSCs) are deemed as the driving force of tumorigenesis in oral squamous cell carcinomas (OSCCs). In this study, we investigated the chemotherapeutic effect of sulforaphane, a dietary component from broccoli sprouts, on targeting OSCC-CSCs. Methods: The effect of sulforaphane on normal oral epithelial cells (SG) and sphere-forming OSCC-CSCs isolated from SAS and GNM cells was examined. ALDH1 activity and CD44 positivity of OSCC-CSCs with sulforaphane treatment was assessed by flow cytometry analysis. In vitro and in vivo tumorigenicity assays of OSCC-CSCs with sulforaphane treatment were presented. Results: We observed that the sulforaphane dose-dependently eliminated the proliferation rate of OSCC-CSCs, whereas the inhibition on SG cells proliferation was limited. Cancer stemness properties including self-renewal, CD44 positivity, and ALDH1 activity were also decreased in OSCC-CSCs with different doses of sulforaphane treatment. Moreover, sulforaphane treatment of OSCC-CSCs decreased the migration, invasion, clonogenicity, and in vivo tumorigenicity of xenograghts. Sulforaphane treatment resulted in a dose-dependent increase in the levels of tumor suppressive miR200c. Conclusion: These lines of evidence suggest that sulforaphane can suppress the cancer stemness and tumor-initiating properties in OSCC-CSCs both in vitro and in vivo.http://www.sciencedirect.com/science/article/pii/S0929664616000280cancer stemnessoral squamous cell carcinomassulforaphane |
| spellingShingle | Chia-Ming Liu Chih-Yu Peng Yi-Wen Liao Ming-Yi Lu Meng-Lun Tsai Jung-Chun Yeh Chuan-Hang Yu Cheng-Chia Yu Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction Journal of the Formosan Medical Association cancer stemness oral squamous cell carcinomas sulforaphane |
| title | Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction |
| title_full | Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction |
| title_fullStr | Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction |
| title_full_unstemmed | Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction |
| title_short | Sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via miR-200c induction |
| title_sort | sulforaphane targets cancer stemness and tumor initiating properties in oral squamous cell carcinomas via mir 200c induction |
| topic | cancer stemness oral squamous cell carcinomas sulforaphane |
| url | http://www.sciencedirect.com/science/article/pii/S0929664616000280 |
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