Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
In bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.
| Váldodahkkit: | , , , , , , , |
|---|---|
| Materiálatiipa: | Artihkal |
| Giella: | English |
| Almmustuhtton: |
Nature Portfolio
2016-11-01
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| Ráidu: | Nature Communications |
| Liŋkkat: | https://doi.org/10.1038/ncomms13350 |