Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing

Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing

Summary: Here, we present a protocol to perform barcode decay lineage tracing followed by single-cell transcriptome analysis (BdLT-Seq). We describe steps for BdLT-Seq experimental design, building barcoded episome reporters, performing episome transfection, and barcode retrieval. We then describe p...

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Bibliographic Details
Main Authors: Yelyzaveta Shlyakhtina, Bianca Bloechl, Katherine L. Moran, Maximiliano M. Portal
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Genomics
Molecular Biology
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007761

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http://www.sciencedirect.com/science/article/pii/S2666166723007761

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