Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells.
Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3847076?pdf=render |
| _version_ | 1818409186843688960 |
|---|---|
| author | Hyejin Tak Md Mamunul Haque Min Jung Kim Joo Hyun Lee Ja-Hyun Baik Youngsoo Kim Dong Jin Kim Regis Grailhe Yun Kyung Kim |
| author_facet | Hyejin Tak Md Mamunul Haque Min Jung Kim Joo Hyun Lee Ja-Hyun Baik Youngsoo Kim Dong Jin Kim Regis Grailhe Yun Kyung Kim |
| author_sort | Hyejin Tak |
| collection | DOAJ |
| description | Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring soluble tau species in living cells. In this regard, we developed a cell-based sensor that visualizes tau self-assembly. By introducing bimolecular fluorescence complementation (BiFC) technique to tau, we were able to achieve spatial and temporal resolution of tau-tau interactions in a range of states, from soluble dimers to large aggregates. Under basal conditions, tau-BiFC cells exhibited little fluorescence intensity, implying that the majority of tau molecules exist as monomers. Upon chemically induced tau hyperphosphorylation, BiFC fluorescence greatly increased, indicating an increased level of tau-tau interactions. As an indicator of tau assembly, our BiFC sensor would be a useful tool for investigating tau pathology. |
| first_indexed | 2024-12-14T09:55:38Z |
| format | Article |
| id | doaj.art-bd6f618cdc974b4dba4e56c7ebeb03e5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T09:55:38Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bd6f618cdc974b4dba4e56c7ebeb03e52022-12-21T23:07:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8168210.1371/journal.pone.0081682Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells.Hyejin TakMd Mamunul HaqueMin Jung KimJoo Hyun LeeJa-Hyun BaikYoungsoo KimDong Jin KimRegis GrailheYun Kyung KimAbnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring soluble tau species in living cells. In this regard, we developed a cell-based sensor that visualizes tau self-assembly. By introducing bimolecular fluorescence complementation (BiFC) technique to tau, we were able to achieve spatial and temporal resolution of tau-tau interactions in a range of states, from soluble dimers to large aggregates. Under basal conditions, tau-BiFC cells exhibited little fluorescence intensity, implying that the majority of tau molecules exist as monomers. Upon chemically induced tau hyperphosphorylation, BiFC fluorescence greatly increased, indicating an increased level of tau-tau interactions. As an indicator of tau assembly, our BiFC sensor would be a useful tool for investigating tau pathology.http://europepmc.org/articles/PMC3847076?pdf=render |
| spellingShingle | Hyejin Tak Md Mamunul Haque Min Jung Kim Joo Hyun Lee Ja-Hyun Baik Youngsoo Kim Dong Jin Kim Regis Grailhe Yun Kyung Kim Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells. PLoS ONE |
| title | Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells. |
| title_full | Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells. |
| title_fullStr | Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells. |
| title_full_unstemmed | Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells. |
| title_short | Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells. |
| title_sort | bimolecular fluorescence complementation lighting up tau tau interaction in living cells |
| url | http://europepmc.org/articles/PMC3847076?pdf=render |
| work_keys_str_mv | AT hyejintak bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT mdmamunulhaque bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT minjungkim bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT joohyunlee bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT jahyunbaik bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT youngsookim bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT dongjinkim bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT regisgrailhe bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells AT yunkyungkim bimolecularfluorescencecomplementationlightinguptautauinteractioninlivingcells |