ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex
ERK 1,2 pathway mediates experience-dependent gene transcription in neurons and several studies have identified its pivotal role in experience-dependent synaptic plasticity and in forms of long term memory involving hippocampus, amygdala or striatum. The perirhinal cortex (PRHC) plays an essential r...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2011-12-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnbeh.2011.00084/full |
| _version_ | 1818050670897397760 |
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| author | Davide eSilingardi Andrea eAngelucci Roberto eDe Pasquale Marco eBorsotti Giovanni eSquitieri Riccardo eBrambilla Elena ePutignano Tommaso ePizzorusso Tommaso ePizzorusso Nicoletta eBerardi Nicoletta eBerardi |
| author_facet | Davide eSilingardi Andrea eAngelucci Roberto eDe Pasquale Marco eBorsotti Giovanni eSquitieri Riccardo eBrambilla Elena ePutignano Tommaso ePizzorusso Tommaso ePizzorusso Nicoletta eBerardi Nicoletta eBerardi |
| author_sort | Davide eSilingardi |
| collection | DOAJ |
| description | ERK 1,2 pathway mediates experience-dependent gene transcription in neurons and several studies have identified its pivotal role in experience-dependent synaptic plasticity and in forms of long term memory involving hippocampus, amygdala or striatum. The perirhinal cortex (PRHC) plays an essential role in familiarity-based object recognition memory. It is still unknown whether ERK activation in PRHC is necessary for recognition memory consolidation. Most important, it is unknown whether by modulating the gain of the ERK pathway it is possible to bidirectionally affect visual recognition memory and PRHC synaptic plasticity.We have first pharmacologically blocked ERK activation in the PRHC of adult mice and found that this was sufficient to impair long term recognition memory in a familiarity-based task, the Object Recognition Task (ORT). We have then tested performance in the ORT in Ras-GRF1 knock-out (KO) mice, which exhibit a reduced activation of ERK by neuronal activity, and in ERK1 KO mice, which have an increased activation of ERK2 and exhibit enhanced striatal plasticity and striatal mediated memory. We found that Ras-GRF1 KO mice have normal short-term memory but display a long term memory deficit; memory reconsolidation is also impaired. On the contrary, ERK1 KO mice exhibit a better performance than WT mice at 72 hour retention interval, suggesting a longer lasting recognition memory. In parallel with behavioural data, LTD was strongly reduced and LTP was significantly smaller in PRHC slices from Ras-GRF1 KO than in WT mice while enhanced LTP and LTD were found in PRHC slices from ERK1 KO mice. |
| first_indexed | 2024-12-10T10:57:10Z |
| format | Article |
| id | doaj.art-bd9281e057004034afaca52145be0fb2 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-12-10T10:57:10Z |
| publishDate | 2011-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-bd9281e057004034afaca52145be0fb22022-12-22T01:51:49ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532011-12-01510.3389/fnbeh.2011.0008415927ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortexDavide eSilingardi0Andrea eAngelucci1Roberto eDe Pasquale2Marco eBorsotti3Giovanni eSquitieri4Riccardo eBrambilla5Elena ePutignano6Tommaso ePizzorusso7Tommaso ePizzorusso8Nicoletta eBerardi9Nicoletta eBerardi10Istituto di Neuroscienze del CNRIstituto di Neuroscienze del CNRIstituto di Neuroscienze del CNRIstituto di Neuroscienze del CNRIstituto di Neuroscienze del CNRUniversità Vita e Salute San RaffaeleIstituto di Neuroscienze del CNRUniversity of FlorenceIstituto di Neuroscienze del CNRIstituto di Neuroscienze del CNRUniversity of FlorenceERK 1,2 pathway mediates experience-dependent gene transcription in neurons and several studies have identified its pivotal role in experience-dependent synaptic plasticity and in forms of long term memory involving hippocampus, amygdala or striatum. The perirhinal cortex (PRHC) plays an essential role in familiarity-based object recognition memory. It is still unknown whether ERK activation in PRHC is necessary for recognition memory consolidation. Most important, it is unknown whether by modulating the gain of the ERK pathway it is possible to bidirectionally affect visual recognition memory and PRHC synaptic plasticity.We have first pharmacologically blocked ERK activation in the PRHC of adult mice and found that this was sufficient to impair long term recognition memory in a familiarity-based task, the Object Recognition Task (ORT). We have then tested performance in the ORT in Ras-GRF1 knock-out (KO) mice, which exhibit a reduced activation of ERK by neuronal activity, and in ERK1 KO mice, which have an increased activation of ERK2 and exhibit enhanced striatal plasticity and striatal mediated memory. We found that Ras-GRF1 KO mice have normal short-term memory but display a long term memory deficit; memory reconsolidation is also impaired. On the contrary, ERK1 KO mice exhibit a better performance than WT mice at 72 hour retention interval, suggesting a longer lasting recognition memory. In parallel with behavioural data, LTD was strongly reduced and LTP was significantly smaller in PRHC slices from Ras-GRF1 KO than in WT mice while enhanced LTP and LTD were found in PRHC slices from ERK1 KO mice.http://journal.frontiersin.org/Journal/10.3389/fnbeh.2011.00084/fullsynaptic plasticityrecognition memory2perirhinal cortexERK1 |
| spellingShingle | Davide eSilingardi Andrea eAngelucci Roberto eDe Pasquale Marco eBorsotti Giovanni eSquitieri Riccardo eBrambilla Elena ePutignano Tommaso ePizzorusso Tommaso ePizzorusso Nicoletta eBerardi Nicoletta eBerardi ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex Frontiers in Behavioral Neuroscience synaptic plasticity recognition memory 2 perirhinal cortex ERK1 |
| title | ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex |
| title_full | ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex |
| title_fullStr | ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex |
| title_full_unstemmed | ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex |
| title_short | ERK pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex |
| title_sort | erk pathway activation bidirectionally affects visual recognition memory and synaptic plasticity in the perirhinal cortex |
| topic | synaptic plasticity recognition memory 2 perirhinal cortex ERK1 |
| url | http://journal.frontiersin.org/Journal/10.3389/fnbeh.2011.00084/full |
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