Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells
A small-size engineered enzyme, ultraID, is presented for proximity-dependent biotinylation, that shows efficient labeling in mammalian cell culture, E. coli and S. cerevisiae.
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-022-03604-5 |