Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

ABSTRACT Whereas 16S rRNA gene amplicon sequencing quantifies relative abundances of bacterial taxa, variation in total bacterial load between samples restricts its ability to reflect absolute concentrations of individual bacterial species. Quantitative PCR (qPCR) can quantify individual species, bu...

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Bibliographic Details
Main Authors: Florencia A. Tettamanti Boshier, Sujatha Srinivasan, Anthony Lopez, Noah G. Hoffman, Sean Proll, David N. Fredricks, Joshua T. Schiffer
Format: Article
Language:English
Published: American Society for Microbiology 2020-04-01
Series:mSystems
Subjects:
applied microbiology
gene amplicon sequencing
quantitative PCR
vaginal microbiome
Online Access:https://journals.asm.org/doi/10.1128/mSystems.00777-19

Internet

https://journals.asm.org/doi/10.1128/mSystems.00777-19

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