Mapping temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coli

Mapping temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coli

Abstract Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each with a single amino acid change in one of 346 essential proteins. 1,269 of these mutants showed temperature‐sensiti...

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Bibliographic Details
Main Authors: Thorben Schramm, Paul Lubrano, Vanessa Pahl, Amelie Stadelmann, Andreas Verhülsdonk, Hannes Link
Format: Article
Language:English
Published: Springer Nature 2023-10-01
Series:Molecular Systems Biology
Subjects:
CRISPR‐Cas9 genome editing
growth‐switches
metabolic valves
metabolomics
temperature‐sensitive mutations
Online Access:https://doi.org/10.15252/msb.202311596

Internet

https://doi.org/10.15252/msb.202311596

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