Use of the 23S rRNA gene as a target template in the universal loop-mediated isothermal amplification (LAMP) of genomic DNA from phytoplasmas

Use of the 23S rRNA gene as a target template in the universal loop-mediated isothermal amplification (LAMP) of genomic DNA from phytoplasmas

ABSTRACTPlant-pathogenic bacteria cause numerous diseases in host plants and can result in serious damage. Timely and accurate diagnostic techniques are, therefore, crucial. While advances in molecular techniques have led to diagnostic systems able to distinguish known plant pathogens at the species...

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Bibliographic Details
Main Authors: Mako Akahori, Akio Miyazaki, Hiroaki Koinuma, Ryosuke Tokuda, Nozomu Iwabuchi, Yugo Kitazawa, Kensaku Maejima, Shigetou Namba, Yasuyuki Yamaji
Format: Article
Language:English
Published: American Society for Microbiology 2024-05-01
Series:Microbiology Spectrum
Subjects:
CTAB
FTA card
on-site detection
ribosomal RNA operon
simple DNA extraction
insect vector
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00106-24

Internet

https://journals.asm.org/doi/10.1128/spectrum.00106-24

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