An improved protocol to study the plant cell wall proteome

An improved protocol to study the plant cell wall proteome

Cell wall proteins were extracted from alfalfa stems according to a 3-steps extraction procedure using sequentially CaCl2, EGTA and LiCl-complemented buffers. The efficiency of this protocol for extracting cell wall proteins was compared with the 2 previously published methods optimized for alfalfa...

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Bibliographic Details
Main Authors: Bruno ePrintz, Raphael eDos Santos Morais, Stefanie eWienkoop, Kjell eSergeant, Stanley eLutts, Jean-Francois eHausman, Jenny eRenaut
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-04-01
Series:Frontiers in Plant Science
Subjects:
Glycosylation
Plant Proteins
Proteomics
EGTA
cell wall proteins
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00237/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00237/full

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